Focal adhesions (FAs) control cell shape and motility, which are important processes that underlie a wide range of physiological functions. FA dynamics is regulated by cytoskeleton, motor proteins and small GTPases. Kinectin is an integral endoplasmic reticulum (ER) membrane protein that extends the ER along microtubules. Here, we investigated the influence of the ER on FA dynamics within the c...

We used optical and X-ray imaging to observe the formation of jets from the impact of a single drop with a deep layer of the same liquid. For high Reynolds number there are two distinct jets: the thin, fast and early-emerging ejecta; and the slow, thick and late-emerging lamella. For low Reynolds number the two jets merge into a single continuous jet, the structure of which is determined by the...

Skin in the tadpole of anuran larvae prior to metamorphosis is composed of two layers of epidermal cells (Leeson and Threadgold, 1961; Chapman and Dawson, 1961; Edds and Sweeny, 1960, 1962), a laminated basement lamella, and a layer of dermal mesenchyme cells apposed to the underside of the basement lamella. An adepidermal space and membrane (Salpeter and Singer, 1959, 1960) separate epidermis ...

The complex blood flow patterns occurring in a trout secondary lamella were computed from a theoretical electrical model consisting of a two-dimensional array of unitary resistances representing the blood channels of the lamella. Estimates of oxygen uptake from water to blood were made by means of the standard conductance equations for oxygen and values from the literature for blood and water P...

We describe a modified technique for loading donor corneal endothelial lamella onto a Busin glide(®) without causing wrinkles, as part of the procedure of Descemet-stripping automated endothelial keratoplasty. Briefly, after punching out a composite of the donor-endothelial lamella and a microkeratome-dissected cap, several drops of dispersive ophthalmic viscosurgical device are placed onto the...

BACKGROUND The transport of labeled G-actin from the mid-lamella region to the leading edge in a highly motile malignant rat fibroblast line has been studied using fluorescence localization after photobleaching or FLAP, and the transit times recorded in these experiments were so fast that simple diffusion was deemed an insufficient explanation (see Zicha et al., Science, v. 300, pp. 142-145 [1]...

PURPOSE To present a technique that creates a neofenestration in the dissecting lamella of chronic aortic dissections using standard or intravenous ultrasound (IVUS)-guided reentry devices. TECHNIQUE To create a neofenestration, a standard or IVUS-guided reentry device is deployed in either the true or false aortic lumen while a balloon is simultaneously inflated in the opposite lumen to stab...

Porokeratotic eccrine ostial and dermal duct nevus (PEODDN) is known as an uncommon disorder of keratinization in which acrosyringium is involved; however, its causes have yet remained unknown. It is presented clinically by discrete keratotic papules on distal extremities arranged in a linear pattern along Blaschko lines. Histopathologically, it is characterized by cornoid lamella, which is alm...

Retinal degeneration slow (RDS/PRPH2) is critical for the formation of the disc/lamella rim in photoreceptor outer segments (OSs), but plays a different role in rods vs. cones. Without RDS, rods fail to form OSs, however, cones lacking RDS (in the rds(-/-)/Nrl(-/-)) exhibit balloon-like OSs devoid of lamellae. We show that distribution of most proteins in the lamella and PM domains is preserved...