The reductions of either acyl or alkyl derivatives of dihydroxyacetone phosphate by enzymes from ascites tumor cell microsomes with NADPH were studied. The reaction rate was measured by determining the formation of labeled lipid from [4-3H]NADPH. The products were characterized as either 1-acyl or 1-alkyl [2-3H]glycerol d-phosphate formed from the corresponding keto derivatives. The enzymes wer...

Pyruvate kinase activity and the rates of gluconeogenesis and glycolysis in rat hepatocytes were evaluated by production of glucose and lactate + pyruvate from dihydroxyacetone during a feeding cycle or progressive starvation. In fed rats, during daylight (low food intake) and until darkness, gluconeogenesis progressively increased and glycolysis decreased slightly, but gluconeogenesis never ex...

At present, a vast majority of products such as plastics, cosmetics or pharmaceuticals are still petroleum-based. A shift towards renewable bio-based feedstock will be inevitable in the future. However, the use of biomass for chemical production implicates the redesign of existing processes and involves new challenges. Handling of aqueous reaction systems and the need for adapted separation tec...

Supported gold nanoparticles play a fundamental role in modern material science and particularly heterogeneous catalysis. are known to be excellent catalysts, not only for the total oxidation of various pollutants (CO, VOCs) but also selective bio-based platform molecules (such as glycerol) added-value chemicals (dihydroxyacetone, glyceric acid, glycolic acid). Controlling their fine dispersion...

We have previously suggested that the effects of alpha 1-adrenergic agents on hepatocyte metabolism involve two mechanisms: (i) a calcium-independent insulin-sensitive process that is modulated by glucocorticoids and (ii) a calcium-dependent insulin-insensitive process that is modulated by thyroid hormones. We have studied the effect of epinephrine (plus propranolol) on gluconeogenesis from lac...

Glycerin-3-P, commonly known as alpha-glycerophosphate and dihydroxyacetone phosphate (DHAP) were measured in well defined microscopic samples of the simple liver acinus allowing a comparison of the glycerin-3-P/dihydroxyacetone-P-ratios of Zones 1 and 3 as a measure of the free NAD+/NADH ratio. The ATP/ADP X Pi quotients were determined in these same microscopic areas of the liver acinus as a ...

Evidence for the formation of an unstable intermediate in the synthesis of quinolinate from aspartate and dihydroxyacetone phosphate by Escherichia coli was obtained using toluenized cells of nadA and nadB mutants of this organism and partially purified A and B proteins in dialysis and membrane cone experiments. The results of these experiments indicate that the nadB gene product forms an unsta...

Analogues of dihydroxyacetone phosphate and of 3-phosphoglycerate were made in which the phosphate group, -O-PO3H2, is replaced by the phosphonomethyl group, -CH2-PO3H2. The analogue of dihydroxyacetone phosphate is a substrate for aldolase and glycerol 1-phosphate dehydrogenase (Stribling, 1974), but not for triose phosphate isomerase. The analogue of 3-phosphoglycerate oxidizes NADH under the...