نتایج جستجو برای: differential scanning
تعداد نتایج: 434473 فیلتر نتایج به سال:
Protein instability is a major obstacle in the production and delivery of monoclonal antibody-based therapies for cancer. This study presents real-time isothermal differential scanning fluorimetry as an emerging method to evaluate the stability of human immunoglobulin G protein with high sensitivity. The stability of polyclonal human immunoglobulin G against urea-induced denaturation was assess...
The equipment for an ultrasensitive, fast, thin-film differential scanning calorimetry @~TDSC! or nanocalorimetry# technique is described. The calorimetric cell ~;0.30 cm! operates by applying a short ~;10 ms! dc current pulse ~;10 mA! to a thin ~;50 nm! patterned metal strip, which is supported by a thin ~;50 nm! SiNx membrane. The calorimeter operates at high heating rates ~15–200 K/ms! and i...
Differential scanning calorimetry has been applied to study factors affecting the thermally induced denaturation of cytoplasmic aspartate aminotransferase, a dimeric pyridoxal enzyme. The consequences of binding of coenzyme and substrate derivatives to both the apo and holo forms of the enzyme were investigated and are interpreted in terms of the stabilization of the native form of the enzyme. ...
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