The mechanism(s) of [35S]sulphate transport was investigated in basolateral liver plasma-membrane vesicles of the little skate elasmobranch, Raja erinacea. Imposition of an intravesicular alkaline pH gradient (pH 8.0 in/pH 6.0 out) stimulated sulphate uptake 5-10-fold compared with pH-equilibrated (pH 8.0 in = out) conditions and 2-3-fold over equilibrium sulphate uptake (overshoot). This pH-gr...

An antibody raised against keratan sulphate was used to localize keratan sulphate in the human embryonic cornea and other human foetal organs. It was found that keratan sulphate was present in the corneal endothelium, as well as in the corneal stroma. In contrast, no keratan sulphate could be demonstrated in the corneal epithelium or in any posterior parts of the human foetal eye. Human corneal...

1. The preparation of potassium l-serylglycine O-sulphate and the corresponding (35)S-labelled ester is described. 2. Intraperitoneal injection of potassium l-serylglycine O[(35)S]-sulphate to rats results in about 75% of the radioactivity of the dose appearing in the urine within 48hr. Almost 72% of the radioactivity recovered in the urine was in the form of inorganic [(35)S]sulphate. 3. Analy...

1. Larvae of Aedes campestris ingest and absorb into their haemolymph large quantities of the sulphate-rich water in which they live, yet they are able to maintain the sulphate content of the haemolymph well below that of the environment. 2. Tracer experiments showed that sulphate regulation was not achieved by deposition of precipitates in the tissues. 3. In vitro preparations of Malpighian tu...

Zinc sulphate and boric acid ophthalmic preparations, containing benzalkonium chloride as preservative, have been investigated against gram-negative and positive bacteria. Viability of bacteria was found after 7 days, although it was completely suppressed after 24 hours of inoculation. Zinc sulphate generally increases the antimicrobial activity of benzalkonium chloride, whereas boric acid ...

Dopamine 3-0-sulphate (3-0-hydrosulphato-4-hydroxyphenethylamine) was isolated from newly ecdysed cockroaches, Periplaneta americana (L.), and its structure established by chemical and physical techniques and by synthesis. Relatively high concentrations (about 1 pmol/g wet wt.) of dopamine 3-0-sulphate exist in the newly ecdysed insect, and these concentrations decrease sharply as sclerotizatio...

Exo-polysaccharides like dextran produced by Weissella sp. have a wide range of applications in the food, pharmaceutical and other industries. This biopolymer and its derivatives like iron dextran, clinical dextran are rapidly emerging as a new and industrially important products. Dextran a polymer of glucose is produced using sucrose rich media with nitrogen source. It also requires efficient ...

13 LOW DOSE MAGNESIUM SULPHATE REGIME FOR ECLAMPSIA Bangal V., Kwatra A., Raghav S., Jadhav S. Abstract Preeclampsia is one of the commonest medical complications seen during pregnancy. It contributes significantly to maternal and perinatal morbidity and mortality. Dr.J.A.Pritchard in 1955, introduced magnesium sulphate for control of convulsions in eclampsia and is used worldwide. Considering ...

1. The mechanism of sulphite and sulphate permeation into rat liver mitochondria was investigated. 2. Extramitochondrial sulphite and sulphate elicit efflux of intramitochondrial phosphate, malate, succinate and malonate. The sulphate-dependent effluxes and the sulphite-dependent efflux of dicarboxylate anions are inhibited by butylmalonate, phenylsuccinate and mersalyl. Inhibition of the phosp...

Human glucuronate 2-sulphatase (GAS), which is involved in the degradation of the glycosaminoglycans heparan sulphate and chondroitin 6-sulphate, was purified almost 2000000-fold to homogeneity in 8 0 yield from liver with a four-step six-column procedure, which consists of a concanavalin A-Sepharose/Blue A-agarose coupled step, a DEAE-Sephacel/octyl-Sepharose coupled step, CM-Sepharose chromat...