Dissertation Cloning and characterisation of a Dictyostelium STE20-like protein kinase DST2 Ehrenwörtliche Versicherung Diese Dissertation wurde selbständig und ohne unerlaubte Hilfsmittel angefertigt. Parts of this work have already been published or are in the process of being published. DST2, a Dictyostelium STE20-like kinase, is phosphorylated by PKA and phosphorylates the actin binding pro...

Actin, an apparently universal component of eukaryotic cells, has been purified from Dictyostelium amoebae to electrophoretic homogeneity. Purification was facilitated by newly discovered solubility properties of actomyosin in 30% sucrose. A quantitative assay for the Dictyostelium actin is described which is based on its ability to activate muscle heavy meromyosin ATPase activity. The specific...

Catechins, flavanols found at high levels in green tea, have received significant attention due to their potential health benefits related to cancer, autoimmunity and metabolic disease, but little is known about the mechanisms by which these compounds affect cellular behavior. Here, we assess whether the model organism Dictyostelium discoideum is a useful tool with which to characterize the eff...

Dimond, R. L., Loomis, W. F. "Acetylglucosaminidase mutants in Dictyostelium discoideum." 1973 Genetics. 74: 64 Cardelli, J. A., Dimond, R. L. "Effect of Starvation and Cell-Cell Interaction on Protein Synthesis in Dictyostelium-discoideum." The 20th Annual Meeting of the American Society for Cell Biology, Cincinnati, Ohio, USA, 1980, J. Cell Biology, 87: (2 Part 2) 275A Knecht, D. A., Dimond, ...

How the collective motion of cells in a biological tissue originates in the behavior of a collection of individuals, each of which responds to the chemical and mechanical signals it receives from neighbors, is still poorly understood. Here we study this question for a particular system, the slug stage of the cellular slime mold Dictyostelium discoideum (Dd). We investigate how cells in the inte...

In amoebae of the eukaryotic microorganism Dictyostelium discoideum, calmodulin is greatly enriched on membranes of the contractile vacuole complex, an osmoregulatory organelle. Antibodies specific for Dictyostelium calmodulin were used in the present study to immunolocalize the contractile vacuole complex in relation to the Golgi complex (detected with wheat germ agglutinin) and the microtubul...

Cell death in the model organism Dictyostelium, as studied in monolayers in vitro, can be induced by the polyketide DIF-1 or by the cyclical dinucleotide c-di-GMP. c-di-GMP, a universal bacterial second messenger, can trigger innate immunity in bacterially infected animal cells and is involved in developmental cell death in Dictyostelium. We show here that c-di-GMP was not sufficient to induce ...

Copyright: © Author, This is an open access article under the terms of the Creative Commons Attribution-NonCommercial No Derives License, which permits use and distribution in any medium, provided the original work is properly cited, the use is noncommercial and no modifications or adaptations are made. Dictyostelium is an eukaryote, a protist, and a slime mold. Dictyostelium shows developmenta...

UNLABELLED With the Dictyostelium Genome Project nearing completion, we initiated the construction of a data repository for all Dictyostelium discoideum genomic data. Up to now this database, called DictyMOLD (Dicty Map Of Linked Data), incorporates the recently completed D.discoideum chromosomes 1 and 2 sequences together with related annotations. To visualise maps, sequences and annotations a...