Chagas disease is caused by the parasite Trypanosoma cruzi. It affects several million people, mainly in Latin America, and severe cardiac and/or digestive complications occur in ~30% of the chronically infected patients. Disease acute stage is mostly asymptomatic and infection goes undiagnosed. In the chronic phase direct parasite detection is hampered due to its concealed presence and diagnos...

OBJECTIVE To conduct an external quality assessment (EQA) of dengue and chikungunya diagnostics among national-level public health laboratories in the Asia Pacific region following the first round of EQA for dengue diagnostics in 2013. METHODS Twenty-four national-level public health laboratories performed routine diagnostic assays on a proficiency testing panel consisting of two modules. Mod...

A. Introduction B. Clinical manifestations of genital herpes 1. Primary genital herpes 2. Non-primary first episodes 3. First episode reactivation 4. Recurrent genital herpes 5. Genital herpes in immunocompromised patients 6. Asymptomatic or unrecognised genital herpes 7. Genital herpes in pregnancy 8. Diagnostic testing of neonates C. Laboratory techniques to detect HSV 1. Viral culture a. Cul...

Asian elephant (Elephas maximus) immunity is poorly characterized and understood. This gap in knowledge is particularly concerning as Asian elephants are an endangered species threatened by a newly discovered herpesvirus known as elephant endotheliotropic herpesvirus (EEHV), which is the leading cause of death for captive Asian elephants born after 1980 in North America. While reliable diagnost...

COVID-19, the infectious pandemic disease is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This deadly was unknown before its catastrophic outbreak of infection in Wuhan city China, December 2019. The situation has increased demand rapid enhancement in-vitro diagnostic assays which would enable mass screening and testing. Several molecular serological diagnostics such ...

Abstract Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, meet this urgent demand, we report a homogeneous immunoassay detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on tri-part Nanoluciferase (tNLuc) approach, in which spike protein of SARS-CoV-2 and G, fused respectively two different tNLuc tags, used as antibody probe...

background and aims: infectious bursal disease (ibd) is an acute contagious viral disease of birds worldwide. the causative virus induces a persistent immune suppression following destroy b lymphocytes precursors in bursal lymphoid follicles. vaccination is the main strategy for prevention of the disease in commercial poultry industry. materials and methods: to produce a live vaccine against th...