We have quantified intraepithelial lymphocytes in the same biopsy series (21 healthy controls, 14 untreated coeliacs, 15 treated coeliacs, five non-coeliac patients with an abnormal jejunal mucosa) both as counts/100 epithelial cells and using as reference value a fixed area of muscularis mucosae. As expected, the number of intraepithelial lymphocytes/100 epithelial cells was significantly high...

Introduction In experimental uveitis, activated g/δT lymphocytes produce cytokines, which contribute to differentiation of pathogenic effector T cells. In addition mucosal tolerance in experimental uveitis can be induced by introducing uveitogenic retinal soluble antigen orally and this phenomenon is dependent on g/δ T cells. The major reservoir for g/δT cells is intestinal mucosa, where they f...

Kinetics of gut lymphocyte subpopulations were studied in normal and malnourished groups of mice infected with Giardia lamblia. The maximum parasite load was observed at second, third, and first week of postinfection in normal controls, moderate (8%) and severe (3%) protein deficient groups respectively. The number of G lamblia trophozoite in 3% protein deficient group was low compared with con...

Lymphocytes of the mouse intestinal mucosa, identified in tissue sections or purified suspensions of intraepithelial lymphocytes as T cells (gut T lymphocytes [GTL]), were studied in normal mice or in beige mice (the equivalent of the Chediak-Higashi syndrome in man, characterized by giant granules in various cell types, including mast cells). Mice were studied in normal or in germ-free conditi...

Submit Manuscript | http://medcraveonline.com Hakansson and Molin [4] suggests that microbial colonization of the GI tract affects the composition of GALT. Once the intestine is exposed to microorganisms, intraepithelial lymphocytes increase; germinal centers with immunoglobulin-producing cells arise rapidly in follicles and in the lamina propria and serum immunoglobulin concentrations increase...

NOD2 functions as an intracellular sensor for microbial pathogen and plays an important role in epithelial defense. The loss-of-function mutation of NOD2 is strongly associated with human Crohn's disease (CD). However, the mechanisms of how NOD2 maintains the intestinal homeostasis and regulates the susceptibility of CD are still unclear. Here we found that the numbers of intestinal intraepithe...

The surface of the human gut has a surplus area of 200-250 m2 in order to contain, between intraepithelial lymphocytes and lamina propria, Peyer’s patches and lymphoid follicles, the lymphoid tissue, while hosts a flora of about 800 different bacteria species with over 7000 strains. The 99% are obligate anaerobes and varies species were then classified using traditional anaerobic culture techni...

In the absence of thymopoiesis, T lymphocytes are nevertheless present, mainly in the gut epithelium. Ontogeny of the extrathymic pathway and the extent of its involvement in euthymic mice are controversial. These questions have been addressed by assessing the expression of recombinase activating gene (RAG) through the use of green fluorescent protein RAG2 transgenic mouse models. In athymic mi...