We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...

membranes to completely remove, replace, or lower the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids, and other biomolecules. The process selectively utilizes permeable (porous) membrane filters to separate the components of solutions and suspensions based on their molecular size. An ultrafiltration membrane retains molecules that are larger than ...

PURPOSE Membrane-based Tangential Flow Filtration (TFF) unit operations are used for clarifying, concentrating, and purifying proteins. This technical brief is a practical introduction to protein processing using tangential flow filtration. It is intended to help scientists and engineers achieve their protein processing objectives by discussing how the choice of key components and operating par...

The direct utilisation of side streams from membrane processing and evaporation is great potential interest for dairies to reduce water consumption. In an analytical screening 89 industrially obtained streams, samples varied in pH conductivity broad ranges 4.22 7.56 0.001 0.949 Siemens per meter, respectively. Detailed compositional data the enabled assessment their applicability as diafiltrati...

The effect of ultrafiltration and diafiltration on the distribution of the calcium phosphate clusters of the casein micelle was investigated using Small Angle Neutron Scattering (SANS). In the case of ultrafiltration, fresh skim milk was subjected to concentration using membrane filtration up to 5× its original volume, the retentate was rediluted with its corresponding serum and subsequently di...

The processing of cheese whey pre-concentrated by reverse osmosis is carried out through ultrafiltration in diafiltration mode to produce whey protein concentrates with lower lactose content to be incorporated in beverages. The initial cheese whey protein and lactose contents are 2.13g/100g and 13.22g/100g, respectively.The commercial membranes, GR95PP, supplied by Alfa Laval, Denmark, we...