The haemolytic activity of iron-dextran complex is found to be a function of time, temperature, pH, and concentration. The lytic action is enhanced by small amounts of added ferrous sulphate. The lytic action is inhibited by chelating agents such as citrate and sequestrene salts, which bind ionic iron, but not by ferric citrate or ferric sequestrene which do not bind iron. The ionised iron cont...

Investigation of the action pattern of enzymes acting on carbohydrates is challenging, as both the substrate and the digestion products are complex mixtures. Dextran and its enzyme-derived oligosaccharides are widely used for many industrial applications. In this work, a new method relying on ultra-performance hydrophilic interaction liquid chromatography quadrupole time-of-flight tandem mass s...

In a previous paper (Ricketts, 1952a) a series of dextran sulphate preparations varying widely in molecular weight and sulphur content was described. The biological properties of these compounds were examined by Walton (1951, 1952), who found that molecular weight was the principal feature determining toxicity, the smaller molecules being the least toxic. At molecular weights (MN) of the order ...

It has recently been recognised that vaccine adjuvants play a critical role in directing the nature of a vaccine induced effector response. In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus antigen, HcsL3. Using a suboptimal antigen dose, aluminium adjuvant was shown to reduce the cumul...

A tissue culture system for detecting rabies virus from saliva samples of suspected animals was developed and compared to suckling mouse inoculation. Swab samples were obtained from the mouth of the animal heads received for rabies diagnosis; these swabs were submerged in maintenance medium. The maintenance medium was inoculated intracerebrally into suckling mice and onto BHK-21 cells with diet...

Three commercially-important types of cell were grown on four different microcarrier substrates. The cells, which included normal human diploid fibroblasts (MRC-5), primary chick embryo cells and Madin-Darby bovine kidney cells (MDBK), were compared with regard to proliferation on the substrates and with regard to recovery of viable cells from the same substrates. The substrates used included g...

We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells ...

Electroporation has proven to be a convenient method for transfection of many cell types. One drawback has been that it requires large amounts of DNA, typically 20 to 80 /tg per transfection (1). Here we report that adding DEAE-dextran to the electroporation media dramatically increases electroporation efficiency in mammalian cells, lowering the amount of DNA required for efficient transfection...

Casein-graft-dextran copolymer was produced using the Maillard reaction. The copolymer is molecularly soluble in neutral aqueous solution whereas beta-carotene is extremely insoluble. By the method of dialysis or evaporation then dispersing in water, the solubility of hydrophobic complex of casein and beta-carotene decreases whereas the solubility of dextran increases gradually, then nanopartic...

Barnes, Isabel J. (Cornell University, Ithaca, N. Y.), H. W. Seeley, and P. J. VanDeMark. Nutrition of Streptococcus bovis in relation to dextran formation. J. Bacteriol. 82:85-93. 1961.-Three nutritional types of Streptococcus bovis were encountered. Minimal media for their growth are described. Transferable growth in defined media was always accompanied by dextran production and the nutrition...