One of the most challenging problems in the development of protein pharmaceuticals is to deal with stabilities of proteins due to its complicated structures. This study aims to develop a novel approach to stabilize and encapsulate proteins into dextran nanoparticles without contacting the interface between the aqueous phase and the organic phase. The bovine serum albumin, granulocyte-macrophage...

Abstract Endothelial progenitor cells (EPCs) have been demonstrated to be effective for the treatment of cardiovascular diseases. However, the differentiation process from circulation to adhesion has not been clarified because circulating EPCs rarely attached to dishes in EPC cultures previously. Here we investigated whether immature circulating EPCs differentiate into mature adhesive EPCs in r...

Dextran sulfate is semi-synthetic, polydisperse sulfated polysaccharide with important applications in clinical practice, in the manufacturing of plasma derived protein therapeutics and in biomedical research. The sensitive detection of dextran sulfate is relevant to preclinical and clinical drug development projects, the quality control of pharmaceutic formulations, and the process control in ...

Abstract Electrospun polyvinyl alcohol–dextran (PVA–Dex)-based nanofibers (NFs) are explored as a novel class of bioactive injury dressing materials, which have an essential role for topical mending. Sodium ampicillin-loaded citric acid-cross-linked PVA–Dex NFs were fabricated by electrospinner wound recuperating purposes. Results revealed that PVA (10%)–dextran (10%) cross-linked with 5% acid ...

The interaction between dextran sulfate and low-density lipoproteins of the Sr 0-10 class in phosphate buffer of pH 7.4 and ionic strength 0.1 was studied by means of analytical ultracentrifugation. The sedimentation pattern of the dextran sulfate-lipoprotein mixture at high dextran sulfate/lipoprotein weight ratios showed a boundary indicative of a soluble complex with a high sedimentation rat...

The hyperglycemic response is an important prognostic factor for survival after hemorrhage. In this study, we investigated the effects of glucose administration during volume resuscitation from hemmorhagic shock in fasted rats under sevoflurane anesthesia on hemodynamics, acid/base-balance and glucose metabolism. Hemorrhagic shock was induced in rats by withdrawing 25 mL/kg of blood. For volume...

Human squamous epithelial cells produce lower amounts of laminin and fibronectin when cultured on DEAE-dextran than when cultured on gelatin-coated polystyrene (Biotechnol. Bioeng., 33:1235). The epithelial cells also spread much more slowly on DEAE-dextran than they do on gelatin-coated polystyrene. To determine if the low level of matrix production by cells grown on DEAE-dextran contributed t...

Dextran a bacterial exopolysaccharide and a long chain polymer of glucose is produced by different microorganisms like Leuconostoc mesenteroides, Lactobacillus spp, Weissella spp and Streptococcus mutans. It has a wide range of application in the food, pharmaceutical and other industries and so is emerging as new and industrially significant product. In the present study the cells of Weissella ...

tuberculosis (tb) and tobacco use are two major alarming global health issues posing immense threats to human populations. mycobacterium tuberculosis (mtb) by activation of macrophages could induce the sequences of cells activation and releases of inflammatory cytokines such as cxcl-8, il-12 and tnf-α which in turn induces the immune system network. however no information is available on other ...

Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as we...