نتایج جستجو برای: denaturing gradient gel electrophoresis

تعداد نتایج: 242037  

F. Lv H. Zhang J. Huang L. Lv Q. Liu, W. Zhao X. Dong Z. Qi

PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...

Journal: :Journal of clinical microbiology 2011
Andreas Matussek Lisa Stark Olaf Dienus Joakim Aronsson Sara Mernelius Sture Löfgren Per-Eric Lindgren

We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2007
Katleen Van der Gucht Karl Cottenie Koenraad Muylaert Nele Vloemans Sylvie Cousin Steven Declerck Erik Jeppesen Jose-Maria Conde-Porcuna Klaus Schwenk Gabriel Zwart Hanne Degans Wim Vyverman Luc De Meester

There is a vivid debate on the relative importance of local and regional factors in shaping microbial communities, and on whether microbial organisms show a biogeographic signature in their distribution. Taking a metacommunity approach, spatial factors can become important either through dispersal limitation (compare large spatial scales) or mass effects (in case of strongly connected systems)....

2011
Jianjun Wang Dongmei Yang Yong Zhang Ji Shen Christopher van der Gast Martin W. Hahn Qinglong Wu

It is widely accepted that biodiversity is lower in more extreme environments. In this study, we sought to determine whether this trend, well documented for macroorganisms, also holds at the microbial level for bacteria. We used denaturing gradient gel electrophoresis (DGGE) with phylum-specific primers to quantify the taxon richness (i.e., the DGGE band numbers) of the bacterioplankton communi...

A.K. Tripathi C.G. Joshi D.N. Rank K.M. Singh M.K. Jhala P.G. Koringa R.A. Mathakiya S.J. Jakhesara V.B. Ahir,

Bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). Populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. The aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...

Dong, X., Huang, J., Liu, Q., Lv, F., Lv, L., Qi, Z., Zhang, H., Zhao, W.,

PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...

2000
Elizabeth Goode David Harlan Wood Junghuei Chen

A model for DNA implementation of Royal Road evolutionary computations is presented. An encoding for a Royal Road problem is presented. Experimental results utilizing 2-d denaturing gradient gel electrophoresis (2-d DGGE) and polyacrylamide gel electrophoresis (PAGE) for separation by tness in this sample Royal Road problem are shown. Suggestions for possible use of the MutS and MutY proteins a...

2017
Xie-feng Yao Jiu-ming Zhang Li Tian Jian-hua Guo

In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting pro...

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