Horse blood has a higher tendency to form red blood cell (RBC) aggregates compared with human blood, with this enhanced aggregation previously attributed to differences in plasma factors. Our results confirm this observation and further indicate that washed horse RBC also have a significantly higher aggregation tendency in dextran 70 solutions (i.e., horse RBC have a higher "aggregability"). In...

Aim This study was aimed to explore the effects of carboxyhemoglobin on reperfusion injury in hemorrhagic shock, as well as its action time and related mechanisms. Results CO-RBC group showed milder oxidative injury than O2-RBC group. CO reperfusion did not show advantages in functions of kidney and lung during resuscitation. The level of Bax was decreased in CO-RBC group, especially in early...

The noncovalent complex formed by the association of two fragments of chymotrypsin inhibitor-2 is reversibly denatured by pressure in the absence of chemical denaturants. On pressure release, the complex returned to its original conformation through a biphasic reaction, with first-order rate constants of 0.012 and 0.002 s-1, respectively. The slowest phase arises from an interconversion of the ...

glucose 6-phosphate dehydrogenase (g6pd) from streptomyces aureofaciens was purified and denatured in 6 m urea. denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...

haemoplasmas contain eperythrozoon and haemobartonella species, which are widespread causes of animal and human infections. these obligatory cell parasites were previously known as hemobartonella; now hemotropic mycoplasmas. these bacteria are identified by the lack of cell wall and their small genome. the usual diameter is between 0.3 and1μm. mycoplasmasarepolymorphic and seen in circular or b...

SCOPE We modeled red blood cell (RBC)-folate response to supplementation and developed personalized folate supplementation concepts. METHODS AND RESULTS The changes of RBC-folate were modeled in a time- (4 or 8 weeks) and dose- (400 or 800 μg d-1 folate) dependent manner. Post-supplementation RBC-folate levels were predicted from folate-loading capacities (= measured RBC-folate - [baseline RB...

The purpose of this study was to investigate red blood cell (RBC) behavior during an increase in local cerebral blood flow (LCBF). We measured changes in RBC behavior by using laser-Doppler flowmetry (LDF) in alpha-chloralose-anesthetized rats. An increase in LCBF was carried out by approximately 2.5 and 4.0% CO(2) inhalation and activation of the somatosensory cortex. The activation of the cor...

BACKGROUND Erythrocyte cholinesterase (RBC-ChE) activities serve as useful and sensitive biomarkers to monitor exposure to cholinesterase-inhibiting substances, such as chemical warfare nerve agents and pesticides. Although the interindividual variation of RBC-ChE is well characterized, the magnitude of intraindividual variation for RBC-ChE remains controversial. An accurate measure of intraind...

BACKGROUND This study aimed at assessing the number of red blood cell (RBC) units transfused at different types of medical institution and examining the characteristics of transfusion recipients. METHODS We calculated and compared the number of transfusion recipients, total RBC units transfused, and RBC units transfused per recipient. Study data were extracted from insurance benefits reimburs...