Sericin is a water-soluble component of silk and has been used as a biomaterial due to its antibacterial and ultraviolet radiation-resistant properties. This study was designed to evaluate the effect of sericin supplementation in a maturation medium on the meiotic competence and fertilisability of sheep oocytes. Cumulus-oocyte complexes (COCs) were cultured in TCM199 supplemented with sericin a...

Tumors inhibit dendritic cell maturation and function in order to evade host immunity. We showed that conditioned media from tumor explant tissue, taken from metastatic colorectal cancer patients, significantly inhibits maturation of dendritic cells.

introduction: standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. this study, therefore, was conducted to assess whether the ...

the study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gff) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in black bengal goats. follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. media were prepared using tcm-199 supplemented wi...

the aim of this study was to determine the quality of cumulus-oocyte-complexes (cocs) and the effects of bovine serum albumin (bsa) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. cocs were collected from slaughterhouse buffalo ovaries by aspiration method. only normal grades cocs were matured for 48 hours in tcm-199 media. two groups were created: one for the ...

This study compared success of in-vitro maturation of rhesus monkey oocytes in protein-free versus serum-containing culture systems, assessed by embryo development subsequent to IVF. Four media were tested: (i) modified Connaught Medical Research Laboratories medium (mCMRL-1066); (ii) hamster embryo culture medium-10 (HECM-10); (iii) control: mCMRL-1066 + 20% bovine calf serum (BCS); (iv) HECM-...

objective: the aim of the present study was to investigate the effect of protein and dna components of toxoplasma gondii on maturation of dendritic cells and their efficiency in il-12 production and proliferation of t cells. materials and methods: for dc generation, bone marrow cells were cultured in the presence of gm-csf and il-4 for 5 days. tumor lysate and protein or dna components of toxo...

Inflammatory mediators in the tumour microenvironment promote tumour growth, vascular development and enable evasion of anti-tumour immune responses, by disabling infiltrating dendritic cells. However, the constituents of the tumour microenvironment that directly influence dendritic cell maturation and function are not well characterised. Our aim was to identify tumour-associated inflammatory m...