The aim of the present study was to investigate the effects of various decellularization methods on the histological and biomechanical properties of rabbit tendons. In total, six chemical reagents, including 1% t-octyl-phenoxypolyethoxyethanol (Triton-X 100), 0.5% sodium dodecyl sulfate (SDS), 1% tri-n-butyl phosphate (TnBP), 1% Triton-X 100 + 0.5% SDS, 1% TnBP + 0.5% SDS and 1% TnBP + 1% Trito...

With the advent of whole organ decellularization, extracellular matrix scaffolds suitable for organ engineering were generated from numerous tissues, including the heart, lung, liver, kidney, and pancreas, for use as alternatives to traditional organ transplantation. Biomedical researchers now face the challenge of adequately and efficiently recellularizing these organ scaffolds. Herein, an ove...

Introduction. Developing a tissue-engineered pancreatic construct (TEPC) involves search for matrices/scaffolds capable of mimicking the structure and composition natural extracellular matrix (ECM), which is an important component tissue microenvironment. A cell-free, tissue-specific obtained from pancreas decellularization seems to be most suitable creation TEPC. The choice protocol should tak...

An attempt has been made to formulate a new method of corneal decellularization using a direct perfusion system through the cornea. Here, we compared the direct perfusion method to some commonly used decellularization strategies including chemical methods; non-ionic detergent TRITON X-100 and ionic detergent; sodium dodecyl sulphate (SDS) based orbital shaker method and physical methods of liqu...

Extracellular matrix (ECM)-based materials are attractive for regenerative medicine in their ability to potentially aid in stem cell recruitment, infiltration, and differentiation without added biological factors. In musculoskeletal tissue engineering, demineralized bone matrix is widely used, but recently cartilage matrix has been attracting attention as a potentially chondroinductive material...

tissue engineering is based on three main factors including scaffolds, cells and growth factors. natural scaffolds derived from decellularized tissues and organs have been successfully used in tissue engineering. decellularization studies have shown that natural scaffolds which maintaine their main structure and properties could be a suitable tool for studying cellular behaviors and preparation...

Decellularization is a process that consists on the removal of immunogenic cellular material from tissue, so it can be safely implanted as functional and bioactive scaffold. Most decellularization protocols rely use harsh chemicals very long washing processes, leading to severe changes in ultrastructure loss mechanical integrity. To tackle these challenges, supercritical carbon dioxide (scCO2) ...

The demand for donated organs greatly exceeds the availability. Alternatives to organ donation, such as laboratory-engineered organs, are therefore being developed. One approach is to decellularize the organ and reseed it with selected cells, ideally from the organ recipient. Organ decellularization has typically been attempted by the administration of detergents into vessels such as the portal...