BACKGROUND Kidneys derived from brain-dead donors have inferior outcomes after transplantation compared to kidneys from living donors. Strikingly, early and profound serum levels of IL-6 in brain-dead donors are observed. IL-6 is the main regulator of the acute phase response (APR). The aim of this translational study was to investigate the expression of renal acute phase proteins (APPs) follow...

Forty-four female students with a tendency for constipation (mean age, 20.2±3.3 y) were asked to consume 30 g test pickles daily for 2 wk and were divided into 3 groups: viable-cell intake subjects (n=14, 3.0×10⁵ colony-forming units of viable LAB (lactic acid bacteria) cells per sample), dead-cells intake subjects (n=15, viable cells were heat sterilized), and placebo-intake subjects (n=15, LA...

An ultrastructural investigation was made of cell death in the uterine epithelium during the implantation of the mouse blastocyst. Cell death occurs in two phases: (i) early individual cell death, and (ii) later general cell death. Individual dead epithelial cells are phagocytosed and digested by trophoblast cells. At 95 h post coitum (p.c.) there are 1-4 dead cells; at 105 h and 113 h, 6-8 dea...

The relationship between the detection of mRNA and cellular viability in Escherichia coli was investigated in cells killed by heat or ethanol. Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH, groEL, and tufA genes. mRNA from all three genes was detected immediately after the cells had been killed by heat or ethanol but gradually disappeared with time when ...

During cell death of human cultured leukocytes (Jurkat, HL-60, THP-1, U937) and freshly prepared leukocytes, we observed a greater than 100-fold increase in the affinity of apoptotic and necrotic cells for fluorescein isothiocyanate (FITC)-heparin in comparison with live cells. Binding of FITC-heparin was reversed in the presence of high ionic strength, unlabeled heparan sulfate, and heparin an...

By NICHOLAS L. PET1t KIS, ESTELLE LIEBERMAN AND JEAN FULLERTON D ISINTEGRATING leukocytes are commonly seen imi blood smears and are variously designated as smudge cells, basket cells, or dead cells. Increased miunibers of these cell forms are found in leukemia and in septic states. The smudge forms are considered by some to represent the earliest stage of cell death amid to be present in the b...

Basic IC fabrication techniques are employed in this paper with the purpose of designing a microfluidic device with a 3D electrode arrangement to separate live and dead biological cells. This is done using the concept of dielectrophoresis, which describes the transnational motion of particles due to the application of a non-uniform electrical field. The simulations where carried out using the p...

Bacillus Sp. was isolated near the mining area and was studied for biosorption U and Th. This study presents uptake of uranium (U) and thorium (Th) by live and dead cells of Bacillus Sp. Increasing concentration of U and Th showed decrease in absorption by Bacillus Sp. Dead cells of Bacillus Sp. exhibited same or more uptake of U and Th than living cells. Increasing temperature promotes uptake ...

Membrane-damaged cells caused by either mechanical trauma or through normal biological processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead cells can nonspecifically bind monoclonal antibody conjugates, potentially leading to erroneous conclusions, particularly when cell frequencies are low. To date, DNA intercalating dyes (Ethidium monoazaide (EMA), Propidium I...