To determine the role of Dp71 in neuronal cells, we generated PC12 cell lines in which Dp71 protein levels were controlled by stable transfection with either antisense or sense constructs. Cells expressing the antisense Dp71 RNA (antisense-Dp71 cells) contained reduced amounts of the two endogenous Dp71 isoforms. Antisense-Dp71 cells exhibited a marked suppression of neurite outgrowth upon the ...

Abstract The native vs. exotic status of reed canarygrass (RCG), a major invasive species Minnesota wetlands, is unknown. aim this study was to investigate enhance its management. Genetic comparison wild RCG populations from six and Czech Republic rivers performed. A total 2521 polymorphic SNP markers (single nucleotide polymorphisms) were used evaluate 478 samples across all collections. In th...

Abstract Its ability to survive under different environmental conditions makes Listeria monocytogenes a critical concern for food safety. When the microorganisms are exposed sublethal heat treatment above their optimum growth temperature, they increase stress adaptation further treatments. In order investigate resistance of L . monocytogenes, L. innocua as surrogate was at 46 °C 30 and 60 min, ...

Several diaminopimelic acid (DAP)- and lysine-requiring mutants of Escherichia coli were isolated and studied by genetic, physiological, and biochemical means. The genes concerned with DAP-lysine synthesis map at several different sites on the E. coli chromosome and, therefore, do not constitute a single operon. Three separate loci affecting DAP synthesis are located in the 0 to 2.5 min region ...

The reported narrow genetic base of cultivated potato (Solanum tuberosum) can be expanded by the introgression of many related species with large genetic diversity. The analysis of the genetic structure of a potato population is important to broaden the genetic base of breeding programs by the identification of different genetic pools. A panel composed by 231 diverse genotypes was characterized...

UNLABELLED Ergosterol is a major and specific component of the fungal plasma membrane, and thus, the cytochrome P450 enzymes (Erg proteins) that catalyze ergosterol synthesis have been selected as valuable targets of azole antifungals. However, the opportunistic pathogen Aspergillus fumigatus has developed worldwide resistance to azoles largely through mutations in the cytochrome P450 enzyme Cy...

leagues describe important findings regarding the development of dilated cardiomyopathy (DCM) and a potential therapeutic option that can alleviate the cardiomyopathic phenotype (1). Using mouse models of cardiomyopathy in which they ablate two of the sarcoglycan complex members, β-sarcoglycan and δsarcoglycan, and thereby disrupt the muscle cytoskeleton and the sarcoglycan-sarcospan complex in...