mitochondrial functions have a major impact on t-cell functionality. in this study we characterized whether mitochondrial function in the neonatal t-cells differs from that in the adult t-cells during short t-cell activation. methods: we used fow cytometry methods to test mitochondrial mass and to monitor mitochondrial ca2+ levels, mitochondrial potential and superoxide generation in parallel w...

Objective: To determine the clinical and laboratory features during disease course in patients of anticytoplasmic antibody (ANCA) associated vasculitis Pakistani presenting to a tertiary care center.
 Study Design: Case series.
 Place Duration Study: Fatima Memorial Hospital Shadman Lahore, from Dec 2018 Mar 2019.
 Methodology: A collection 20 regarding demographic data, constitu...

Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...

Our previous studies revealed an inhibitory effect of ICD-85 (Venom derived peptides) on breast cancer cell line MDA-MB231. ICD-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. However, the exact mechanism of ICD-85 was unknown. Hence, the present study was undertaken to assess the mechanism of ICD-85 effect as an anti-proliferative agent of cancer cells. The effec...

Background and Objective: Adenocarcinoma of the prostate is the second most common cause of cancer. The loss of CD10 is a common early event in human prostate cancer and is seen in lower Gleason Score malignancies while increased and altered expression is seen in high Gleason Score tumors, lymph nodes and bone metastasis. Material and Methods: This was a ...

Background Exfoliation of the surface cells normal epithelium occurs as a result physiological turnover. Epithelial are constantly renewed, and they exfoliated or shed migrate from basal layer to uppermost epithelium. Oral exfoliative cytology involves collection microscopic evaluation these scraped epithelial cells, quantitatively qualitatively. The objective present study was analyze compare ...