While there are many different methods for peak detection, no automatic marking boundaries to calculate area under the curve (AUC) and signal-to-noise ratio (SNR) estimation exist. An algorithm automation of liquid chromatography tandem mass spectrometry (LC–MS/MS) chromatogram quantification was developed validated. Continuous wavelet transformation other digital signal processing were used in...

To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness. Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile enterotoxin (LT I) and th...

Abbreviations: %CV: Coefficient of Variation; Ae: Amount Excreted; AE: Adverse Event; AUC: Area under the Plasma Concentrationversus-Time Curve; AUC0-∞: Area under the Concentration-time Curve from Time 0 to Infinity; Cl/F: Apparent Oral-Dose Clearance; CLR: Renal Clearance; Cmax: Peak Plasma Concentration; CYP: Cytochrome P450; ECG: Electrocardiogram; LC/MS/MS: Liquid Chromatography/Tandem Mas...

PURPOSE To explore the contribution of flow cytometry immunophenotyping (FCI) in detecting leptomeningeal disease in patients with solid tumors. EXPERIMENTAL DESIGN Cerebrospinal fluid (CSF) samples from 78 patients who received a diagnosis of epithelial-cell solid tumors and had clinical data suggestive of leptomeningeal carcinomatosis (LC) were studied. A novel FCI protocol was used to iden...

BACKGROUND Laparoscopic surgery for common bile duct stones varies procedurally from a transcystic approach to laparoscopic choledochotomy (LC) with or without biliary drainage. However, LC is a difficult procedure with higher documented morbidity than the transcystic approach. We retrospectively investigated risk factors for adverse outcomes of LC. METHODS We used logistic regression models ...

Bakcground: Laparoscopic cholecystectomy (LC) is generally accepted as a standard treatment of cholelythiasis. However there are trends to further minimize surgical trauma and to improve cosmetic results by performing LC through a single transumbilical incision. The aim of this study was to evaluate the learning curve and early results of transumbilical laparoscopic cholecystectomy (TC) perform...

PURPOSE High levels of serum-soluble mesothelin family proteins (SMRP) have been found to be associated with malignant mesothelioma (MM), but not lung cancer (LC). To verify the clinical role of this marker for both these tumors, we tested serum SMRP in the largest population of thoracic cancers ever assembled. EXPERIMENTAL DESIGN SMRP blood concentrations were measured in 107 patients with M...

Hot water extracts of Ginkgo biloba seeds were analyzed for the presence of ginkgotoxin (4'-O-methylpyridoxine) by reversed-phase liquid chromatography (LC) using methanol-0.05M KH2PO4 (1 + 9, v/v) adjusted to pH 3 as mobile phase. Detection was by fluorescence (excitation 280 nm, emission 370 nm). A straight line calibration curve was obtained for the 10-100 ng injected. After addition of beta...

In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Immunoreactive TH on PVDF membranes was identified with optimized concentrations...

BACKGROUND Cotinine has been widely used as an objective marker to identify current smokers. We conducted this study to address the absence of Korean studies investigating the efficacy of immunoassays and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the detection of serum cotinine and to determine the optimal serum cotinine cut-off level for differentiating current smokers from...