Microbiology laboratories continually strive to streamline and improve their urine culture algorithms because of the high volumes of urine specimens they receive and the modest numbers of those specimens that are ultimately considered clinically significant. In the current study, we quantitatively measured the impact of the introduction of CHROMagar Orientation (CO) medium into routine use in t...

The aminoglycoside antibiotics, particularly gentamicin, are increasingly widely used and frequently in patients with impaired renal function. All these drugs are potentially ototoxic and measurement of blood levels is often desirable but may be complicated by the presence of a second drug, most commonly a penicillin or cephalosporin. Assays of streptomycin in the presence of penicillin have be...

Study question: Does the type of medium used to culture fresh and frozen-thawed embryos influence neonatal birthweight after single embryo transfer (SET) in IVF? Summary answer: A comparison of two commercially available culture media showed no significant influence on mean birthweight and mean birthweight adjusted for gestational age, gender and parity (z-scores) of singletons born after a fre...

This case report illustrates the need for regular review of culture methods and maintenance of thorough attempts at diagnosis prior to treatment of gonorrhoea. Vancomycin-sensitive gonococcal infection was discovered by repeated tests and by using a non-selective medium (locally). The support of the Gonococcus Reference Unit confirmed the findings. The observations are discussed in laboratory, ...

A technique is described by which a medium composed of fibrinogen suspension, serum, and embryo juice may be made. Fibroblasts grew in this medium about as well as in plasma and embryo juice. A strain of connective tissue in this medium remained practically as active as the control for several passages.

The chemically defined culture media for Pasteurella pestis which were formulated by Rao (1939, 1940a, b), Berkman (1942), Doudoroff (1943), and Herbert (1949) did not support the growth of any of the 27 strains available in this laboratory. This report describes the.development of a chemically defined medium which was evaluated in terms of the following criteria: yields of viable cells, minimu...

Classic methods of biosurfactant separation are difficult and require large amounts of organic solvents, thus generate high amounts of waste. This work presents and discusses in detail an original procedure to separate rhamnolipid from fermentation broth using high performance membrane techniques. Due to the unique properties of surface active agents, such as capability of forming aggregates ab...

Acton el al. (1932) used a vegetable medium from a green variety of mung dal (Phaseolus mungo) for cultivation of the intestinal bacteria. Brewer (1943) suggested a mixture of soyabean and cotton seed or peanut meal for the preparation of a general purpose media using sodium sulphide as an activator; the sodium sulphide would readily generate sulphuretted hydrogen which by lowering the pH of th...

Neurobasal defined culture medium has been optimized for survival of rat embryonic hippocampal neurons and is now widely used for many types of primary neuronal cell culture. Therefore, we were surprised that routine medium exchange with serum- and supplement-free Neurobasal killed as many as 50% of postnatal hippocampal neurons after a 4 h exposure at day in vitro 12-15. Minimal Essential Medi...