Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation for precipitation of bacterial pellets, and subsequent lysis of the pellets. This process is time-consumin...

India is the largest producer of rice (Oryza sativa L.) next to China in the world. Rice cultivation is a huge water demanding enterprise. In recent years, water table has depleted at a very rapid rate throughout the globe, thus, posing alarming threats and limiting the scope for cultivation of high water consuming crops especially rice. Challenges in maintaining the sustainability of rice farm...

In general, morphological, physiological, and biochemical properties of fungal cells are drastically different among solid-state (SSC), liquid-surface (LSC), and submerged cultivation (SmC) systems. In addition to these traditional cultivation systems, a unique liquid-surface cultivation [liquid-surface immobilization (LSI)] system and interface cultivation systems [extractive liquid-surface im...

in the country, reducing water resources and increasing water losses in the various methods of surface irrigation, has increased attention, super drip irrigation method, especially drip tape irrigation. one of the techniques are used in cultivation of early sweet corn, the use of plastic mulch. using this technique, the reserve temperature conditions for plant growth and early maturity, resulti...