Troponin I, a subunit of the ternary troponin complex, is a regulatory protein of the thin filament of striated muscle. Cardiac troponin I (cTnI) is uniquely different from the troponin I found in either slow or fast skeletal muscle. Possessing an additional 31-amino acid sequence at its N terminus, cTnI is found exclusively in the myocardium and, therefore, has been extensively studied as a se...

AIMS To evaluate the prevalence of cardiac troponin I (cTnI) elevation in an elderly community population and the association of cTnI levels with cardiovascular risk factors, vascular inflammation, atherosclerosis, cardiac performance, and areas indicative of infarcted myocardium identified by cardiac magnetic resonance imaging. METHODS AND RESULTS cTnI elevation defined as cTnI levels >0.01 ...

OBJECTIVES High sensitivity cardiac troponin T and I (hs-cTnT and hs-cTnI) assays show analytical, diagnostic and prognostic improvement over contemporary sensitive cTn assays. However, given the importance of troponin in the diagnosis of myocardial infarction, implementing this test requires rigorous analytical and clinical verification across the total testing pathway. This was the aim of thi...

BACKGROUND Patients with aneurysmal subarachnoid haemorrhage (SAH) are at risk of cardiac and pulmonary complications. Troponin I (cTnI), a reliable marker of myocardial injury, is frequently raised after SAH. AIMS To investigate the additional value of (cTnI) in predicting cardiac or pulmonary complications and outcome in patients with SAH. METHODS Admission cTnI was measured in a prospect...

BACKGROUND The prevalence of subclinical, cardiac troponin I (cTnI) elevation in stable patients undergoing elective percutaneous coronary intervention and its relationship to culprit lesion characteristics assessed by optical coherence tomography (OCT) are unknown. METHODS AND RESULTS We studied 206 native de novo culprit coronary lesions from 206 patients with stable angina pectoris who und...

OBJECTIVES This study evaluated the analytical characteristics of the Liaison immunoassay for cardiac troponin I (cTnI). DESIGN AND METHODS The protocol consisted of eight sections: evaluation of antibody specificity, linearity, detection limit and imprecision, method comparison, evaluation of endogenous interferents, anticoagulant interference, sample stability, and reference values. RESUL...

To determine the forms of cardiac troponin I (cTnI) circulating in the bloodstream of patients with acute myocardial infarction (AMI) and patients receiving a cardioplegia during heart surgery, we developed three immunoenzymatic sandwich assays. The first assay involves the combination of two monoclonal antibodies (mAbs) specific for human cTnI. The second assay involves the combination of a mA...

This paper describes the detection of a cardiac biomarker, cardiac troponin I (cTnI), spiked into depleted human serum using cationic isotachophoresis (ITP) in a 3.9 cm long poly(methyl methacrylate) (PMMA) microfluidic channel. The microfluidic chip incorporates a 100 crosssectional area reduction, including a 10 depth reduction and a 10 width reduction, to increase sensitivity during ITP. The...

BACKGROUND Cardiac troponin-I (cTNI) is highly specific biomarker to prove myocardial damage, e.g. in acute coronary syndrome (ACS). However, it occurs in other conditions as well. We therefore analysed cTNI increase in patients after generalized convulsive seizure. METHODS Consecutive patients admitted with acute generalized convulsive seizure were included in case of cTNI measurement on adm...