Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and...

Background: Medical diagnostic procedures such as X-ray and computed Tomography (CT) scan account for considerable percent of patient's exposure to ionizing radiation. The exposure of cells to Ionization radiation results in induction of DNA damage and chromosomal aberrations. Contrast media (CM) are widely used in diagnostic radiology and CT scan. The aim of this study was to study adverse gen...

Gene therapy is a technique that currently under expansion and development. Recent advances in genetic medicine have paved the way for broader range of therapies laid groundwork next-generation technologies. A terminally substituted difluorene-diester Schiff Base calix[4]arene has been studied this work as possible nanovector to be used gene therapy. Changes luminescent behavior calixarene macr...

Electrochemical DNA-based sensors that exploit the inherent sensitivity of DNA-mediated charge transport (CT) to base pair stacking perturbations are capable of detecting base pair mismatches and some common base damage products. Here, using DNA-modified gold electrodes, monitoring the electrocatalytic reduction of DNA-bound methylene blue, we examine a wide range of base analogues and DNA dama...

Background: Coumarins comprise a large family of heterocyclic compounds with benzo-a-pyrone moiety. Objectives: This study aimed to analyze the binding affinity 3-(1H-tetrazol-5-yl) coumarin bovine serum albumin (BSA) and calf thymus DNA (Ct-DNA) using fluorescence spectroscopy. The quenching was recognized during interaction between BSA, followed by static mechanism. Methods: hydrogen bonds, h...

DNA charge transport (CT) chemistry has received considerable attention by scientific researchers over the past 15 years since our first provocative publication on long range CT in a DNA assembly.1,2 This interest, shared by physicists, chemists and biologists, reflects the potential of DNA CT to provide a sensitive route for signaling, whether in the construction of nanoscale biosensors or as ...

The role of base motions in delocalization and propagation of charge through double helical DNA must be established experimentally and incorporated into mechanistic descriptions of DNA-mediated charge transport (CT). Here, we address these fundamental issues by examining the temperature dependence of the yield of CT between photoexcited 2-aminopurine (Ap) and G through DNA bridges of varied len...