This research investigated the Eucalyptus cinerea leaves efficiency in the Agrobacterium tumefaciens biocontrol, the causative agent of crown gall. GC-MS analysis of the essential oil (EO) showed that the main components were 1,8-cineole (61%) and camphene (15.13%). Thanks to its polyphenols, flavonoids, quinones, terpenoids, alkaloids, and tannins richness, the EtOAc-F exhibited the most poten...

Rabbits were injected with cell walls obtained from crown-gall tumor tissue or the corresponding cell walls from normal potato tissue. The serum obtained from rabbits 53 days after they were injected with tumor cell walls contained immunoglobins that reacted with both tumor and normal cell walls as well as with the cells from the inciting strain of Agrobacterium tumefaciens. When this serum was...

Crown gall of walnut (Juglans sp.), caused by the bacterium Agrobacterium tumefaciens, greatly impacts the CA walnut industry. To determine the genetic diversity of A. tumefaciens throughout the Central Valley of CA, we collected isolates from ten walnut growing counties. A total of 340 A. tumefaciens biovar 1 isolates were collected and tested for the presence of the Ti plasmid by PCR; 190 iso...

Cross-reacting antigens were found in bacteria-free crown gall tumor tissue tested with serum prepared against Agrobacterium tumefaciens (Smith and Towns.) Conn., but no such antigens were detected in callus tissue. Soluble proteins from tumor tissue, callus tissue, and the crown gall bacteria were fractionated on a DEAE-Sephadex (A-50) column. The diethylaminoethyl-Sephadex elution profile for...

Several years ago, Robison and Robison (5) reported the synthesis of some compounds of biological interest. Among these were 7-aza-indole-3-acetic acid (AIAA) and 7-aza-indole-3-propionic acid (AIPA), aza analogues of indole-3-acetic acid (IAA), and indole-3-propionic acid (IPA). It was apparent that such compounds might be anti-metabolites of auxin, in the same manner that azaguanine has prove...

Advances made recently in the study of plant growth substances and their function in normal growth have raised the question of their possible role in atypical and pathological growth and specifically in the gall development induced by Phytomonas tumefaciens (Smith and Town.) Bergey et al. A suggestion of their presence was first given by the marked epinasty of leaf petioles in plants inoculated...

Virulent Agrobacterium tumefaciens strains integrate their T-DNA into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. Essential for crown gall development are IaaH (indole-3-acetamide hydrolase), IaaM (tryptophan monooxygenase) and Ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (IaaH, IaaM) and cytokinin (...