New semi-interpenetrating polymeric network (IPN) hydrogels based on chitosan and poly(acrylic acid), crosslinked with glutaraldehyde, were prepared by free radical polymerization method. IPN hydrogels were synthesized by simultaneous polymerization/crosslinking of acrylic acid monomer in the presence of chitosan and crosslinker. The structure and surface morphology of the IPN hydrogels were ch...

Characterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the p...

Aspects of the molecular-level basis for the function of ethylene glycol dimethacrylate and trimethylolproprane trimethacrylate crosslinked methacrylic acid copolymers molecularly imprinted with (S)-propranolol have been studied using a series of all-component and all-atom molecular dynamics studies of the corresponding prepolymerization systems. The crosslinking agents were observed to contrib...

The present study is aimed at developing novel type of interpenetrating polymeric network (IPN) hydrogels of chitosan with poly(acrylic acid) for the controlled release of amphetamine drug. IPN hydrogels were synthesized by simultaneous polymerization/crosslinking of acrylic acid monomer in the presence of chitosan and crosslinker. The swelling properties and in vitro drug release characteristi...

Gelatins were crosslinked with organic acids and treated with alkali to impart to them endothelialization and anti-thrombogenic properties. These matrices were characterized by biochemical and physicochemical techniques. The amounts of residual amino groups in the matrices decreased with increasing crosslinker concentration. The matrices with the highest crosslinking densities showed excellent ...

In this study, the corn straw after removing the lignin was grafted with 2-acrylamido-2-methylpropanesulfonic acid (AMPS) to prepare sulfonated cellulose. The grafting copolymerization between the sulfonated cellulose and acrylic acid (AA) was performed using potassium persulfate and N,N1-methylenebisacrylamide as the initiator and crosslinking agent, respectively, to prepare corn straw-co-AMPS...

Molecular imprinting is a new and rapidly evolving technique used to create synthetic receptors and it possesses great potential in a number of applications in the life sciences. Keeping in mind the therapeutic importance of 5-fluorouracil (5-FU) and the technological significance of molecular imprinting polymers, the present study is an attempt to synthesize 2-hydroxyethylmetacrylate- and acry...

Article history: Received January 26, 2013 Received in Revised form May 10, 2013 Accepted 20 May 2013 Available online 24 May 2013 Superabsorbent hydrogels based on poly(vinyl alcohol) were prepared by a crosslinking technique using glutaraldehyde as a crosslinker. The hydrogel structure was confirmed using scanning electron microscopy (SEM). Results from SEM observation showed a porous structu...

During cytokinesis, global and equatorial pathways deform the cell cortex in a stereotypical manner, which leads to daughter cell separation. Equatorial forces are largely generated by myosin-II and the actin crosslinker, cortexillin-I. In contrast, global mechanics are determined by the cortical cytoskeleton, including the actin crosslinker, dynacortin. We used direct morphometric characteriza...

Sorting of target cells from a heterogeneous pool is technically difficult when the selection criterion is complex, e.g. a dynamic response, a morphological feature, or a combination of multiple parameters. At present, mammalian cell selections are typically performed either via static fluorescence (e.g. fluorescence activated cell sorter), via survival (e.g. antibiotic resistance), or via seri...