UNLABELLED PREMISE OF THE STUDY This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • METHODS AND RESULTS Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 ma...

background and objective: streptococcosis/lactococcosis is the cause of high morbidity and mortality in aquaculture sector and to date a number of species of streptococcus and lactococcus genera including s. iniae, s. agalactiae, s. dysagalactiae, s. parauberis, s. feacalis, l. garvieae and l. lactis have been discriminated as the cause of disease in aquatic animals. despite the use of diagnost...

common kilka clupeonella cultriventris (nordmann, 1840) is a brackish water and small pelagic fish species and is one of the most abundant fishes that live gregariously in the caspian sea. a total of 60 specimens of adult common kilka were sampled from two seasons. fifteen pairs of microsatellites previously developed for a.sapidissima, c. pallasi, c.harengus, and s. pilchardus were tested for ...

Background: Due to the predisposing conditions in patients with cystic fibrosis (CF) caused by defective mucociliary clearance facilitating colonization and invasion with Candida species has dramatically increased. Traditional methods for identifying problems are imminent and time-consuming. Therefore, molecular techniques utilizing amplification of target DNA provide quick and precise methods ...

Vibrio parahaemolyticus (V. parahaemolyticus) is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. In the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (MCDA-LFB) to detect the toxR gene of V. parahaemolyticus in DNA extracts from pure cultures and spiked oyster homogenates. Amplification was carried out...

The grass Puccinellia maritima is an important saltmarsh ecosystem engineer exhibiting wide morphological variation, which is partially genetically determined. Nevertheless, nothing is known about its population genetics or how neutral genetic variation is distributed throughout its geographical range. Here, we describe 12 polymorphic microsatellites pooled into two multiplexes for this octoplo...

BACKGROUND Sturnira is one of the most species-rich genera in the Neotropics, and it is found from Mexico and the Lesser Antilles to Argentina. This genus forms a well-supported monophyletic clade with at least twenty-one recognized species, as well as several others under taxonomic review. Sturnira parvidens is a widespread frugivorous bat of the deciduous forests of the Neotropics, is highly ...

A PCR-based ‘molecular tool box’, based on a region of the ras-related protein gene Ypt 1, was developed for the identification of 15 Phytophthora species that damage forests and trees: P. cactorum , P. cambivora , P. cinnamomi , P. citricola , P. europaea , P. inundata , P. lateralis , P. megasperma , P. nemorosa , P. kernoviae , P. pseudosyringae , P. psychrophila , P. quercina , P. ramorum a...