penicillin g acylase from e. coli ta1 was immobilized by cross-linked enzyme aggregates (clea), a new method for immobilization. this biocatalyst and commercial immobilized penicillin g acylase (pga-450) were used to study the effect of ph, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (pgme) and 6-aminopenicillanic acid (6-apa). compare...

Objective. The objective was to compare the postprandial glycemic and insulinemic responses to nutrition bars containing either cross-linked RS type 4 (RS4(XL)) or standard wheat starch in normoglycemic adults (n = 13; age = 27 +/- 5 years; BMI = 25 +/- 3 kg/m(2)). Methods. Volunteers completed three trials during which they consumed a glucose beverage (GLU), a puffed wheat control bar (PWB), a...

Abstract In the drilling industry, demand for environmentally friendly additives with high thermal stability is increasing due to dual factors of environmental pressure and high-temperature oil layers. However, commonly used non-toxic biodegradable additives, such as etherified modified starch, cannot withstand temperatures higher than 150 °C. Additionally, natural polymers better obtained thro...

Plantain (Musa paradisiaca) starch,  prepared as crosslinked starch-urea (CSU), was utilized in the formulation of matrix tablets ambroxol hydrochloride. CSU synthesized by gelatinization plantain starch urea, with calcium chloride. Native and were characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), chemical material properties. Ambroxol hydrochloride prepared CSU, ...

The production of porous cross-linked enzyme aggregates (p-CLEAs) is a simple and effective methodology for laccase immobilization. A three-phase partitioning technique was applied to co-precipitate laccase and starch, followed by cross-linking with glutaraldehyde and removal of starch by α-amylase to create pores in the CLEAs. Scanning electron microscopy revealed a very smooth spherical struc...