Our knowledge of the evolution and role untranslated region (UTR) in SARS-CoV-2 pathogenicity is very limited. Leader sequence, originated from UTR, found at 5? ends all encoded transcripts, highlighting its importance. Here, leader sequence was compared between human pathogenic non-pathogenic coronaviruses. Then, profiling microRNAs that can inactivate key UTR regions coronaviruses carried out...

uterine infection is one of the reproductive diseases that can have disturbing postpartumuterine health in cattle. therefore, identification of resistant genotypes to uterine infection isimportant. lactoferrin (lf) is one of the major antimicrobial compounds in the normal uterinedischarges of cows. we hypothesized that allelic diversity in lf gene may contribute tosusceptibility or resistance t...

rapid detection and differentiation of infectious bronchitis virus (ibv) involved in the disease outbreak is very important for controlling disease and developing new vaccines. in the present study, three regions of the genome of ibv vaccine and field isolates including s1 gene, gene 3 and nucleocapsid (n) gene along with 3' untranslated region (3' utr) were amplified and subjected to restricti...

The immune checkpoint HLA-G prevents maternal rejection of the fetus and contributes in cancer invasion and acceptance of allografts. The 5' and 3' regulatory regions of the HLA-G gene are polymorphic and balancing selection probably maintains this variability. It is proposed that nucleotide variations may affect the level of HLA-G expression. To investigate this issue we aimed to analyze how h...

hla-g is a non-classical hla class ib molecule with limited protein variability generated by  alternative  splicing.  hla-g   displays  immunotolerant   properties  and  hence  plays important roles in the maintenance of a successful pregnancy and maternal tolerance of the semiallogenic fetus. polymorphism of the hla-g gene may potentially affect the biological properties of the protein, and a ...

BACKGROUND The morbidity of erectile dysfunction (ED) has been found to be substantially increased in patients with chronic prostatitis (CP). Accumulating evidence shows that single-nucleotide polymorphism (SNP) located in pre-miRNA or mature microRNA may affect the processing of microRNA (miRNA) and alter the expression of the miRNA, as well as its target gene. In this study we investigated th...

We describe here a one pot RNA production, packaging and delivery system based on bacteriophage Qβ. We demonstrate a method for production of a novel RNAi scaffold, packaged within Qβ virus-like particles (VLPs). The RNAi scaffold is a general utility chimera that contains a functional RNA duplex with paired silencing and carrier sequences stabilized by a miR-30 stem-loop. The Qβ hairpin on the...