BACKGROUND Corynebacterium glutamicum is generally regarded as a safe microorganism and is used to produce many biochemicals, including L-glutamate. 5-Aminolevulinic acid (ALA) is an L-glutamate derived non-protein amino acid, and is widely applied in fields such as medicine and agriculture. RESULTS The products of the gltX, hemA, and hemL genes participate in the synthesis of ALA from L-glut...

Corynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. Although the Streptococcus pyogenes (Sp) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into C. glutamicum. Here we report a Francisella novicida (Fn) CRISPR-Cpf1-based genome-editing method for C. glutamicum. CRISPR-Cpf1, com...

soil salinity induces a stressful environment for soil micro-organisms and reduces their number and activity. the objective of this research is investigating the effect of different levels of salinity on some of soil biological indicators and also assessing the effect of two types of halotolerant rhizospheric bacteria on the value of these indexes in soil. 35 strains were isolated from saline r...

Relatively limited information about promoter structures in Corynebacterium glutamicum has been available until now. With the aim of isolating and characterizing such transcription initiation signals, random Sau3A fragments of C. glutamicum chromosomal DNA and of the corynebacterial phage phi GA1 were cloned into the promoter probe vector pEKplCm and selected for promoter activity by chloramphe...

Abstract A simplified and scalable one‐pot process for the anaerobic production of succinic acid using a metabolically engineered Corynebacterium glutamicum strain is demonstrated. With targeted bioprocess optimization, titer 78 g L −1 yield 1.41 mol SA GLC were achieved. Succinic was recovered from neutral fermentation broth by electrochemically induced crystallization applied polybutylene bio...

Previous studies with Corynebacterium diphtheriae and Mycobacterium species revealed that the transcriptional regulator DtxR and its ortholog IdeR play a central role in the control of iron metabolism. In the present work, we used genome-based approaches to determine the DtxR regulon of Corynebacterium glutamicum, a nonpathogenic relative of C. diphtheriae. First, global gene expression of a dt...

The Corynebacterium glutamicum ('Brevibacterium lactofermentum' AJ12036) odhA gene, encoding 2-oxoglutarate dehydrogenase (E1o subunit of the 2-oxoglutarate dehydrogenase complex), has been isolated and identified as an homologous counterpart of the Escherichia coll sucA and Bacillus subtilis odhA genes. The nucleotide sequence of a 4394 bp chromosomal fragment containing the C. glutamicum odhA...

Involvement of the Global Regulator GlxR in 3-Hydroxybenzoate and Gentisate 1 Utilization by Corynebacterium glutamicum 2 3 Hongjun Chao and Ning-Yi Zhou 4 5 Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of 6 Virology, Chinese Academy of Sciences, Wuhan 430071, China 7 8 Running title: Global Regulator GlxR for 3-HBA and GEN catabolism 9

Lipopolysaccharides free P[3-hydroxybutyrate (3HB)-co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3HV unit accumulated 8-47wt% of P(3HB-co-3HV). The 3HV fraction in the copolymer was varied from 0 t...

Accessible online at: www.karger.com/cpb SIGNIFICANCE OF THE CORYNEBACTERIUM GLUTAMICUM MSCCG MECHANOSENSITIVE CHANNEL IN FINE-TUNING OF COMPATIBLE SOLUTE ACCUMULATION Kirsten Börngen1, Andrew R. Battle2,3, Nina Möker1, Susanne Morbach1, Kay Marin1, Boris Martinac3 and Reinhard Krämer1 1University of Cologne, Köln, Germany, 2Griffith University, Parklands, Queensland, Australia, 3Victor Chang C...