The purpose of our review was to familiarize the readers with the new concepts in ocular surface diseases and reconstruction. Limbal stem cell deficiency is characterized by the progressive invasion of conjunctival epithelial cells onto the cornea, superficial vascularisation, destruction of the corneal basement membrane, and chronic inflammatory cell infiltration. Depending on the severity of ...

chemical injuries of the cornea are ophthalmic emergencies. these injuries often result in significant ocular morbidity and generally strike young adults in the prime years of life. classification schemes enable the ophthalmologist to determine the severity of injury and the prognosis for the injured eye. immediate management consists of copious irrigation following exposure. effective manageme...

Purpose. To compare the surgically induced astigmatism from clear corneal and square posterior limbal incisions at the time of cataract surgery. Methods. Surgically induced astigmatism was calculated for a set of eyes after cataract surgery using a temporal 2.2 mm square posterior limbal incision. Results were compared to similar available data from surgeons using clear corneal incisions of sim...

PURPOSE To determine if vitamin A could be one of the factors in serum responsible for the previously observed effect of 20% fetal bovine serum on stimulating clonal growth of an additional subpopulation in limbal cultures, possibly stem cells, but inhibiting that of transient amplifying cells in peripheral corneal cultures. METHODS A reported serum-free clonal growth assay was used. The mito...

Peripheral human corneal epithelium grows better in tissue culture than central epithelium, but it is not known whether ocular limbal epithelium grows even better than does the peripheral corneal epithelium. In this work we compared the growth kinetics of limbal and peripheral human corneal epithelial cells in tissue culture. Four 1-2 mm2 explants, removed from the limbus or from peripheral cor...

PURPOSE Limbal fibroblasts are known to regulate the maintenance and differentiation of the corneal epithelium including the limbal epithelial stem cells. This study examined the effect of limbal fibroblast conditioned media in a mouse model of limbal stem cell deficiency. METHODS Limbal stem cell deficiency was created in C57/Bl6 mice by performing a limbus to limbus epithelial debridement. ...

The human cornea requires a smooth, transparent, robust and renewable surface to maintain its key optical and protective functions. The corneal epithelium is maintained by a local population of “stem cells”, which although not truly pluripotent, are capable of self-regeneration by asymmetric division. These cells are located at the limbus the region where the conjunctiva-covered sclera meets th...

The human cornea requires a smooth, transparent, robust and renewable surface to maintain its key optical and protective functions. The corneal epithelium is maintained by a local population of “stem cells”, which although not truly pluripotent, are capable of self-regeneration by asymmetric division. These cells are located at the limbus the region where the conjunctiva-covered sclera meets th...

PURPOSE To study the expression and cellular distribution of multiple S100A genes and proteins in normal corneal-limbal epithelium and ocular surface squamous cell carcinoma (SCC) tissue. METHODS Normal corneal-limbal tissue was obtained from the Lions Eye Bank, Tampa, FL. Ocular surface SCC tissues were excised from patients undergoing surgery at Singapore National Eye Centre. S100A mRNA exp...

PURPOSE To investigate morphologic alterations in the limbal palisades of Vogt in a progressive form of limbal stem cell deficiency. METHODS Twenty Norwegian subjects (40 eyes) with congenital aniridia and 9 healthy family members (18 eyes) without aniridia were examined. Clinical grade of aniridia-related keratopathy (ARK) was assessed by slit-lamp biomicroscopy, and tear production and qual...