PURPOSE Bacterial lipopolysaccharide (LPS, endotoxin) is a potent stimulator of inflammatory responses and is likely to contribute to microbial keratitis and to the pathogenesis of sterile corneal ulcers. The purpose of the present study was to identify specific mediators of endotoxin-induced keratitis. METHODS The corneal epithelium of BALB/c, C3H/HeJ, and C3H/HeN mice was abraded, and Pseud...

PURPOSE An increase in corneal temperature has been implicated in subepithelial haze after surface photorefractive keratectomy. We aimed to determine if cooling the cornea by means of hydration between ablations and immediately after final surface ablation in PRK reduces haze. METHODS One hundred five eyes of 61 patients with myopia ranging from -6.00 to -9.75 D were included. One of the surg...

BACKGROUND Hevin is a matricellular protein involved in tissue repair and remodeling via interaction with the surrounding extracellular matrix (ECM) proteins. In this study, we examined the functional role of hevin using a corneal stromal wound healing model achieved by an excimer laser-induced irregular phototherapeutic keratectomy (IrrPTK) in hevin-null (hevin(-/-)) mice. We also investigated...

This study examined the effects of BMP7 gene transfer on corneal wound healing and fibrosis inhibition in vivo using a rabbit model. Corneal haze in rabbits was produced with the excimer laser performing -9 diopters photorefractive keratectomy. BMP7 gene was introduced into rabbit keratocytes by polyethylimine-conjugated gold nanoparticles (PEI2-GNPs) transfection solution single 5-minute topic...

determinants of early onset haze were retrospectively explored in patients who had been undergone laser-assisted sub-epithelial keratectomy (lasek) in a private eye clinic. the technique included creating an epithelial flap following 20 seconds exposure to 20% alcohol. ‘optimized’ ablation profile was used by allegretto laser (wavelight technologie). following repositioning of the flap, a singl...

The corneal sensibility was examined with the aesthesiometer of Draeger in 41 patients after refractive corneal surgery, 31 patients after radial keratotomy, 5 after epikeratophakia, 5 after excimer laser ablation. It could be shown that after radial corneal incisions the sensibility remains normal. After epikeratophakia the corneal sensibility is asensible even 3 years after operation. The len...

The aim of the present study was to determine the effect of different concentrations of mitomycin C (MMC) administered intraoperatively during laser-assisted subepithelial keratectomy (LASEK) surgery. A total of 261 patients (496 eyes) were randomly divided into two groups: 0.04% MMC treatment group consisting of 133 patients (245 eyes) and the 0.02% MMC treatment group consisting of 128 patien...

orneal haze is the most serious complication of photorefractive keratectomy (PRK) and has been attributed to normal healing processes. 1-3 The prediction of which patients are likely to develop haze is of the utmost importance in the evaluation of patients for PRK. We noted that while removing the epithelium prior to corneal ablation, three distinct types of cornea can be appreciated: 1) normal...

Over the years, mitomycin C has been used by refractive surgeons to prophylactically decrease haze after surface ablation procedures and therapeutically in the treatment of preexisting haze. Development of mitomycin C treatments has had a significant role in the revival of surface ablation techniques. We reviewed the literature regarding mechanism of action of mitomycin C, its role in modulatin...