Endothelial cell loss is a key indicator of the quality anterior segment surgery like cataract surgery. The amount and integrity corneal endothelial cells are two most essential factors determining transparency. Aim this study was to compare between superior scleral tunnel incision temporal clear for phacoemulsification. Prospective observational study. This prospective included 50 patients wit...

Human corneal endothelial cells (HCEnCs) form a monolayer of hexagonal cells whose main function is to maintain corneal clarity by regulating corneal hydration. HCEnCs are derived from neural crest and are arrested in the post-mitotic state. Thus cell loss due to aging or corneal endothelial disorders leads to corneal edema and blindness-the leading indication for corneal transplantation. Here ...

purpose: to assess the efficacy and safety of laser in situ keratomileusis (lasik) for correction of residual myopia and astigmatism after penetrating keratoplasty (pk). methods: in this prospective interventional case series, lasik was performed on 14 eyes of 13 patients unable to wear glasses or contact lenses after pk. the interval between lasik and pk was at least one year. all patients had...

PURPOSE To assess the risk factors for endothelial cell loss after phacoemulsification with implantation of intraocular lens according to anterior chamber depth (ACD). METHODS This prospective study included 94 eyes of 94 patients undergoing phacoemulsification cataract surgery. To assess the risk factors for corneal endothelial cell loss, we examined seven variables at 1 day, 1 week, 6 weeks...

purpose: to evaluate the effect of human amniotic fluid (haf) on the growth of human corneal endothelial cells (hcecs) and to establish an in vitro method for the expansion of hcecs. methods: hcecs were cultured in dmem-f12 supplemented with 20% fetal bovine serum (fbs). confluent monolayer cultures were trypsinized and passaged using 20% fbs- or 10%, 20%, or 30% haf-containing media. cell prol...

Corneal endothelial dysfunction is a major cause of visual impairment, since these cells maintain corneal transparency. Although surgical techniques have been developed to replace abnormal corneal endothelium, these procedures are technically difficult and challenging due to a shortage of donor corneas. Regenerative medicine uses specially grown tissues to replace injured ones and has been succ...

OBJECTIVE To compare the long-term effects of dorzolamide hydrochloride (Trusopt, Merck and Co Inc, White-house Station, NJ), timolol maleate, and betaxolol hydrochloride on corneal endothelial cell density and corneal thickness. METHODS This 1-year multicenter study was conducted in 298 patients with ocular hypertension or open-angle glaucoma who had a baseline central corneal endothelial ce...

BACKGROUND Wound healing of the endothelium occurs through cell enlargement and migration. However, the peripheral corneal endothelium may act as a cell resource for the recovery of corneal endothelium in endothelial injury. AIM To investigate the recovery process of corneal endothelial cells (CECs) from corneal endothelial injury. METHODS Three patients with unilateral chemical eye injurie...

PURPOSE To investigate cell surface-associated keratan sulfate on the corneal endothelium. METHODS Immunolabeling techniques were used at the light, scanning, and transmission electron microscopic level to localize keratan sulfate on the corneal endothelium. The investigation included human, bovine, and rabbit corneal endothelia. A quantitative study of the relationship between cell size and ...