AIM To evaluate different thawing temperatures and duration on the post-thaw semen quality of Indian yaks bulls. MATERIALS AND METHODS Semen ejaculates from four different yak bulls were collected using artificial vagina method and extended with tris extender containing 6.4% glycerol at 35°C, cooled gradually from 35°C to 5°C at 1°C/3 min and equilibrated at 4-5°C for 4 h and frozen in French...

Aim: The aim was to determine the antioxidative capacity of vitamin E, vitamin C and their combination (vitamin E+C) on standard semen parameters i.e., mortality, percent live sperms, percent abnormal sperms and acrosomal integrity in cryopreserved Bhadawari bull semen. Materials and Methods: Ejaculates collected from four Bhadawari bulls were evaluated and later pooled at 37°C. Pooled semen sa...

The purpose of this study was to investigate the efficiency of the water test to evaluate the functional membrane integrity of ejaculated canine sperm after collecting, cooling, and freezing-thawing. Twenty-five ejaculates were obtained from 12 stud dogs by digital manipulation. Sperm-rich fractions were evaluated, diluted, cooled at 5oC and frozen-thawed. A conventional hypo-osmotic swelling t...

The effect of gelatin addition to the semen extender on the viability and acrosome integrity of rabbit spermatozoa was studied. Pooled semen samples were processed in a boar semen extender with or without gelatin addition. Semen samples were stored at 5 degrees C for 72 h. Viability and acrosome integrity was evaluated by light microscope. Results showed that gelatin addition had a significant ...

The sperm quality index (SQI) is predictive of fresh semen quality. Our objective was to examine if semen storage affects the SQI obtained from undiluted semen, or semen diluted with either Beltsville Poultry Semen Extender (BPSE) or Minimum Essential Medium (MEM) and held for 8 h at 4, 21, or 41 C. Dead o sperm percentage was higher and SQI was lower from undiluted versus diluted semen. Dead s...

this experiment was conducted to evaluate the addition of seminal plasma to coated spermatozoa after 72 h. semen was collected from four taleshi rams by artificial vagina. to prepare coated spermatozoa, semen was collected in tube containing tris-fructose-15% egg yolk. after initial evaluation, the samples were mixed and centrifuged. after removal of supernatant and dilution, samples were divid...

The Punjab Urial (Ovis vignei punjabiensis) is an endangered subspecie of ovidae, distributed as small scattered populations in the forest belt of the Himalayan foothills of Pakistan and in the areas enclosed by the Indus and the Jhelum rivers. The present study was conducted to evaluate the liquid storage of Punjab Urial spermatozoa in different extenders for use in future in situ conservation...

________________________________________________________________________________ Abstract The study evaluated the effect of different cryoprotectants on post-thaw survival and motility of Kolbroek sperm. Semen from Kolbroek boars was collected with the gloved hand technique. Ejaculates were diluted with Beltsville thawing solution (BTS) at a ratio of 1 : 1 prior to freezing. Semen was diluted w...

The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen store...

An artificial insemination (AI) program using cooled semen was evaluated over a 7-year period in Florida goats. The effect of the following variables on pregnancy rates was analysed: production system, year and season of AI, synchronisation treatment, bucks, AI technicians, semen deposition site, days in milk at AI, milk yield and parity. Animals were reared under field conditions on commercial...