نتایج جستجو برای: conventional culture method
تعداد نتایج: 2069477 فیلتر نتایج به سال:
Conventional cytogenetic is the standard technique for detection of Philadelphia (Ph) chromosome in chronic myeloid leukemia (CML). Evaluation of abelson murine leukemia/breakpoint cluster region (abl/bcr) fusion using dual-colour fluorescence in situ hybridization (D-FISH) is an alternative approach allowing rapid and reliable detection of the disease. We employed the technique of interphase D...
In the present research work, a specific 285 bp DNA fragment was used for detection of Mycobacterium Tuberculosis complex. 100 samples were chosen randomly from sputum specimens that were negative with conventional methods (direct smear, culture, and radiometry), and examined by PCR 7 cases of them were positive. Also, 20 sputum specimens were obtained from suspected patients to tuberculosis, a...
in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...
BACKGROUND Infectious diseases are a leading cause of morbidity and mortality in developing countries. The aim of this study was to compare the results of blood culture employing the conventional and BACTEC methods for detection of bacterial infection in Taleghani Hospital, Tehran. MATERIALS AND METHODS This was a descriptive study carried out for 3 months (March 2014-May 2014) on 272 inpatie...
Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture techni...
BACKGROUND Nosocomial infection is one of the most common complications within health care facilities. Certain studies have reported outbreaks resulting from contaminated hospital environments. Although the identification of bacteria in the environment can readily be achieved using culturing methods, these methods detect live bacteria. Sequencing of the 16S ribosomal RNA (16S rRNA) gene is reco...
BACKGROUND AND OBJECTIVES Fungal endophthalmitis is a devastating form of ocular infection and postoperative endophthalmitis following cataract surgery is the commonest form of such infection. Early diagnosis is very important for effective management of these patients. As conventional techniques take longer time and lacks sensitivity, polymerase chain reaction (PCR) for detection of fungal DNA...
The conventional virus isolation technique was compared with a 24-h shell vial centrifugation culture technique and with a 48-h tube culture method for the detection of cytomegalovirus (CMV) in MRC-5 cells. Of 200 clinical specimens tested, 41 were positive for CMV by at least one procedure. Indirect immunoperoxidase staining was positive for 32 (78.0%) of 41 specimens in the tube culture metho...
A rapid, sensitive and cost-effective method was developed for detection of foodborne pathogens, particularly Salmonella species. The method utilizes single stranded DNA (ssDNA) probes and non-functionalized gold nanoparticles to provide a colorimetric assay for the detection of PCR amplified DNA. Different food samples were tested with the PCR-based colorimetric assay parallel with the convent...
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