Abstract Background Measurement of multiple steroids, 17 hydroxyprogesterone, 11 deoxycortisol, and 21 is required to discriminate between congenital adrenal hyperplasia due hydroxylase deficiency that beta deficiency. This work aims at the selection more appropriate, cost-effective method among either mass spectrometry or radioimmunoassay for quantitation previous steroids. In this study, bloo...

Barnes, N. D., and Atherden, S. M. (1972). Archives of Disease in Childhood, 47, 62. Diagnosis of congenital adrenal hyperplasia by measurement of plasma 17-hydroxyprogesterone. Measurement of plasma 17-hydroxyprogesterone by a simple competitive protein-binding assay has proved of value in the diagnosis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency. Children with untreated...

Congenital adrenal hyperplasia refers to a group of autosomal recessive disorders caused by a deficiency of an enzyme involved in the synthesis of glucocorticoids. The enzyme deficiency generally leads to a deficiency of cortisol and/or aldosterone production within the adrenal cortex. The lack of glucocorticoids generally leads to elevated levels of plasma corticotropin (ACTH), which often res...