Silver-enhanced labeling method has been employed in immunochromatographic assays for improving the sensitivity of detecting pathogens. In this paper, we apply the silver enhancement technique for biomolecular signal amplification in a gold nanoparticle-based conductimetric biochip. We show that the response of the silver-enhanced biochip comprises two distinct regions namely: (a) a sub-thresho...

The electrolytic conductivities for C.I. Acid Orange 15 and C.I. Acid Red 97 have been studied. The electrolytic conductivities have been analyzed by Deby HUckel-Onsager theory, The degree of ionization, α, Van’t Hoffs factor, i, and thickness of ionic atmosphere, X –1 . were calculated. Each value diminishes with increasing dye concentration. The dye anion transport number, t, mobility’s, μ we...

Complex formation between a natural polysaccharide – gellan and an antimicrobial drug ofloxacin was studied in aqueous solution. Conductimetric potentiometric titration curves revealed that forms water-soluble complex of composition 2:1 mol/mol stabilized by ionic hydrogen bonds. The the gellan-ofloxacin confirmed FTIR spectroscopy, dynamic light scattering, zeta-potential thermogravimetric ana...

In order to characterize the ion-exclusion and cation-exchange properties of an unmodified silica-gel column, the retention behaviors of Cr(VI) and Cr(III) ions were investigated using a Develosil 30-5 (150 x 4.6 mm i.d.) in the acidic region. Cr(VI) was separated from other anions by an ion-exclusion and ion-adsorption mechanism, and Cr(III) was separated from other cations with a cation-excha...

A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range ...

Biosensors based on electrochemical sensors for multiple analytes were the first scientifically proposed as well as successfully commercialized biosensors.A long time study has been done for electrochemical biosensors.A typical platform for the construction of biosensors is represented by transducers based on semiconductors and screen printed electrodes.The most common biorecognition components...

A smpile, rapid and accurate method for the determination of main mono and divalent inorganic anions in Roselle (Hibiscus sabdariffa L.) has been developed. The quantitative determination of anions F-, Cl-, NO2, Br ,NO3, HPO 24,SO 24, and I was accomplished by suppressed conductimetric ion chromatography (IC). The separation is achieved on shim-pack IC-SA2 250 mmL X 4.0 mm ID Peak. column opera...

We developed an ion-exclusion/adsorption chromatography (IEAC) method employing a polystyrene-divinylbenzene-based weakly acidic cation-exchange resin (PS-WCX) column with propionic acid as the eluent for the simultaneous determination of multivalent aliphatic carboxylic acids and ethanol in food samples. The PS-WCX column well resolved mono-, di-, and trivalent carboxylic acids in the acidic e...