Our work has focused on the determination of physiological traits that may facilitate in situ degradation of xenobiotic compounds by indigenous microorganisms. For this our interests center on the following questions: What are the ambient conditions in a benzene, toluene, ethylbenzene, and xylene (BTEX)-contaminated aquifer? What is the behavior of indigenous bacteria under these conditions? Wh...

The soil fungus Cladophialophora sp. strain T1 (= ATCC MYA-2335) was capable of growth on a model water-soluble fraction of gasoline that contained all six BTEX components (benzene, toluene, ethylbenzene, and the xylene isomers). Benzene was not metabolized, but the alkylated benzenes (toluene, ethylbenzene, and xylenes) were degraded by a combination of assimilation and cometabolism. Toluene a...

An indirect competitive chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) for the determination of benzene, toluene, ethylbenzene, and ortho-, meta-, para-xylenes (BTEX) in soil and water was developed. The assay was optimized concerning the coating conjugate concentration, anti-BTEX antiserum dilution, incubation time effect on primary and secondary antibody incubation, and temper...

Over the three-day gasification test of a large coal block with oxygen in atmospheric pressure conditions, yield and composition tar collected was investigated. The sampled approximately every 7 h into sorption tubes directly from reactor outlet. Sand, moisture content 11%, used as an insulating material to simulate environment gasified seam. Light aromatic hydrocarbons (BTEX), phenols, polycyc...

The need for gas compound measurement concerns overall three domains: environmental monitoring, emission measurement and risk assessment. These fields are different because of concentration range (from 10–3 to thousands mg.m–3). A fast technique has been developed based on UV spectrophotometry. Simple robust optics and absence of interference from water vapour and carbon dioxide are two of the ...

Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the P. putida strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocar...

M. Holtzer, B. Grabowska, S. Żymankowska-kumon, D. Kwaśniew skakrólikowska, R. Dańko, W. Solarski, A. Bobrowski, AGH University of Science and Technology, Faculty of Foundry Engineering, Kraków, Poland Procedures have been developed to determine the volume, rate and composition (particularly BTEX: benzene, toluene, ethylbenzene and xylenes and PAHs (polycyclic aromatic hydrocarbons)) of gas evo...

S. Żymankowska-Kumon, AGH University of Science and Technology, Kraków, Poland The article contains the results of emission BTEX (benzene, toluene, ethylobenzene and xylenes) during thermal destruction (500 – 1 300 °C) of moulding sand with furan resin. This furan resin is with a high content of free furfuryl alcohol (about 80 %). Knowledge of emissions of these compounds allows the assessment ...

The degradation of benzene, toluene, ethylbenzene and xylene (BTEX) contaminants in groundwater relies largely on anaerobic processes. While the physiology and biochemistry of selected relevant microbes have been intensively studied, research has now started to take the generated knowledge back to the field, in order to trace the populations truly responsible for the anaerobic degradation of BT...