Three of at least 8 Fanconi anemia (FA) genes have been cloned (FANCA, FANCC, FANCG), but their functions remain unknown. Using the yeast 2-hybrid system and full-length cDNA, the authors found a strong interaction between FANCA and FANCG proteins. They also obtained evidence for a weak interaction between FANCA and FANCC. Neither FANCA nor FANCC was found to interact with itself. These results...

Defining the subcellular distribution of signaling complexes is imperative to understanding the output from that complex. Conventional methods such as immunoprecipitation do not provide information on the spatial localization of complexes. In contrast, BiFC monitors the interaction and subcellular compartmentalization of protein complexes. In this method, a fluororescent protein is split into a...

Complementation and determinization are two fundamental notions in automata theory. The close relationship between the two has been well observed in the literature. In the case of nondeterministic finite automata on finite words (NFA), complementation and determinization have the same state complexity, namely Θ(2n) where n is the state size. The same similarity between determinization and compl...

We prove that, with very few exceptions, every graph of order n, n = 0, 1 (mod 4) and size a t most n 1, is contained in a self-complementary graph of order n. We study a similar problem for digraphs. Throughout the paper, G and D will denote a finite graph and a finite digraph, respectively, without loops or multiple edges, with vertex-sets V(G) and V ( D ) , and edge-sets E(G) and E(D); defin...

Nine lethal complementation groups flanking the Drosophila Dopa decarboxylase (Ddc) gene, have been localized within 100 kb of cloned chromosomal DNA. Six of these complementation groups are within 23 kb of DNA, and all ten complementation groups, including Ddc, lie within 78-82 kb of DNA. The potential significance of this unusually high gene density is discussed.

Part of the FA complex with FANCC, FANCE, FANCF, and FANCG; this complex is only found in the nucleus. FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This FA complex translocates into the nucleus, where FANCE and...