The tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), induced specific changes in the cell surface glycoprotein profile of K562 human leukemic cells. TPA-treated K562 cells were examined by cell surface labeling followed by immunoprecipitation with anti-glycophorin antiserum or anti-K562 antiserum. TPA-treated K562 cells lost glycophorin and glycoprotein with a molecula...

BACKGROUND AND PURPOSE Tissue-type plasminogen activator (tPA) is the only drug approved for the acute treatment of ischemic stroke but with two faces in the disease: beneficial fibrinolysis in the vasculature and damaging effects on the neurovascular unit and brain parenchyma. To improve this profile, we developed a novel strategy, relying on antibodies targeting the proneurotoxic effects of t...

The metabolism of the tumor promoters 120-O-[3H]tetradecanoylphorbol-13-acetate ([3H]TPA) and [3H]phorbol-12,13-didecanoate ([3H]PDD) was analyzed in several cell types in culture. In contrast to the rapid metabolism of [3H]TPA, [3H]PDD was degraded much more slowly in hamster, rat, chick, and mouse fibroblasts. Human fibroblasts did not significantly metabolize either phorbol diester over a th...

Exercise has been linked to a reduced cancer risk in animal models. However, the underlying mechanisms are unclear. This study assessed the effect of exercise with dietary consideration on the phospholipid profile in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse skin tissues. CD-1 mice were randomly assigned to one of the three groups: ad libitum-fed sedentary control; ad libitum-fed...

The present study was aimed to evaluate the protective effects of Hydroethanolic Murraya koenigii leaves extract (HEMKLE) against DMBA/TPA-induced angiogenesis, hepatotoxicity and renal toxicity during skin carcinogenesis in mice. For study, male LACA mice were divided into four groups: Group I (C), II (DMBA/TPA), III (HEMKLE), IV (HEMKLE+DMBA/TPA). 7, 12-dimethylbenz(a) anthracene (DMBA), 12-O...

A high-density array of plasmonic coaxial nanoantennas is used to enhance the two-photon absorption (TPA) process in a conventional silicon photodetector from a mode-locked 76 MHz Ti:sapphire laser over a spectral range from 1340 to 1550 nm. This enhanced TPA was used to generate an interferometric autocorrelation trace of a 150 fs laser pulse. Unlike second-harmonic generation, this technique ...

BACKGROUND Marine sponge species are of significant interest to many scientific fields including marine ecology, conservation biology, genetics, host-microbe symbiosis and pharmacology. One of the most intriguing aspects of the sponge "holobiont" system is the unique physiology, interaction with microbes from the marine environment and the development of a complex commensal microbial community....

hemorrhagic side effects of tPA. It is tempting to speculate that administration of FXIII might decrease tPA-induced hemorrhage by restoring FXIIIa that has been inactivated by plasmin, but further studies are necessary to test this hypothesis. Conflict-of-interest disclosure: Oregon Health & Science University and D.H.F. have a significant interest in Gamma Therapeutics, a company that may hav...

The purpose of this research project is to identify and chemically profile active extracts of a marine sponge, Clathria sp., collected from St. Thomas, US Virgin Islands. Previously, pteridine alkaloids, sterol sulfates, and bromine-containing amides collected from various species of Clathria have shown anti-microbial and anti-cancer activities [1]. Sponge extracts were isolated through a combi...

in this research, a novel formulation of sponge cake was studied. instant tea powder (itp) with high ester-catechins content was used to replace 0, 7.5, 12.5, and 17.5% of flour to make sponge cakes, hereafter referred to as the control, itp1, itp2, and itp3, respectively. the microbiological analysis and lipid oxidation experiments were conducted and the odor, flavor, color, chewiness, and ove...