Coupling capillary columns in gas chromatography (GC) is not an operation that is only relevant for the users of the most advanced capillary GC methods. Simple single-column separations can also benefit from a properly selected and installed pre-column. Pre-columns connected upstream of the main column can increase the life-time of the column, efficiently refocus the analyte bands to result in ...

the isolation and characterization of two new isoquinoline alkaloids, dehydropuntarenine and dehydrosaulatine, obtained during the column chromatography of an alkaloidal fraction from the roots of b. actinacantha (berberidaceae) are described.

New antibiotics designated decatromicins A and B were isolated from the culture broth of Actinomadura sp. MK73-NF4. They were purified by butyl acetate extraction, silica gel column chromatography, silica gel TLC and Sephadex LH-20 column chromatography. Decatromicins A and B inhibited growth of Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA).

A novel procedure was developed for rapid separation of the three component enzymes of pig heart 2-oxoglutarate dehydrogenase complex by high performance liquid chromatography on a gel filtration column. The complex was dissociated and separated into two fractions of the first dihydrolipoamide succinyltransferase and a second yellow fraction within 1 h by chromatography on a preparative TSK-GEL...

The isolation and characterization of two new isoquinoline alkaloids, Dehydropuntarenine and Dehydrosaulatine, obtained during the column chromatography of an alkaloidal fraction from the roots of B. Actinacantha (Berberidaceae) are described.

We have succeeded in isolating virus from the particle laden solution like body fluid. Because many of virus infectious diseases occur in the developing region, compact and low-cost device for virus detection from the patients’ body fluid is required. We proposed a microfluidic chip for virus isolation by hydroxyapatite chromatography. This microfluidic chip utilized a column and a pair of swit...

P reparative chromatography is the dominant purification technique in the production of biological compounds, especially for bioseparations. The column used is central to the performance of the chromatographic step. To ensure robust and reproducible column performance, columns need to be qualified in an acceptable fashion before each use. Some of the critical elements of column qualification in...

A toxin that causes morphological changes in Chinese hamster ovary cells was isolated from a culture filtrate of Shigella dysenteriae 1. It was partially purified by successive column chromatography on diethylaminoethyl-cellulose, Bio-Gel A-5m, and hydroxylapatite. The toxin was separated from neurotoxin by diethylaminoethyl-cellulose column chromatography and was not lethal for mice.