Introduction: In the point of physical view, there are no significant differences between tumor and normal tissues during radiation therapy. Radio-sensitizers have a key role to address the issue. Exploiting high atomic number, gold nanoparticles (GNPs) have been introduced as novel radio-sensitizers and have shown promising result in the field. Owing to high mass attenuation c...

BACKGROUND Iron overload is associated with liver toxicity, cirrhosis, and hepatocellular carcinoma in humans. While most iron circulates in blood as transferrin-bound iron, non-transferrin-bound iron (NTBI) also becomes elevated and contributes to toxicity in the setting of iron overload. The mechanism for iron-related carcinogenesis is not well understood, in part due to a shortage of suitabl...

The clonogenic or colony formation assay is a widely used method to study the number and size of cancer cell colonies that remain after irradiation or cytotoxic agent administration and serves as a measure for the anti-proliferative effect of these treatments. Alternatively, this assay is used to quantitate the transforming potential of cancer associated genes and chemical agents. Therefore, th...

In vitro radiation survival of peripheral blood T lymphocytes was studied in 15 clinically normal adults and 4 patients with Fanconi's anemia. Tritiated thymidine incorporation in a whole blood lymphocyte stimulation test (LST) and a newly developed whole blood T-lymphocyte colony assay were used to measure lymphocyte blastogenesis and colony formation in response to phytohemagglutinin (PHA) or...

Abstract Radiotherapy resistance is a challenge for colorectal cancer (CRC) treatment. Circular RNAs (circRNAs) play vital roles in the occurrence and development of CRC. This study aimed to investigate role circ_0005615 regulating radiosensitivity The levels circ_0005615, microRNA-665 (miR-665), notch receptor 1 (NOTCH1) were detected by quantitative real-time PCR or western blot. CRC cells wa...


 Purpose: To investigate the effect of microRNA-612 (miR-612) on human colon cancer cells, and mechanism involved.
 Methods: Expressions miR-612 GPR132 were determined by quantitative real-time polymerase chain reaction (qRT-PCR)el , while cell viability was evaluated using counting kit-8 (CCK8) colony formation assays. Dual luciferase assay used to determine interaction between GPR1...

Supplemental Experimental Procedures Production of virus. Cell culture and human iPS cell induction. Donor blood cells collection and isolation. Immunohistochemistry staining. qRT–PCR. Global gene expression. Bisulfite genomic sequencing. DNA fingerprinting and karyotyping. Embryoid body formation. Hematopoietic colony-forming assays. Assay for teratoma formation. Flow cytometry. Southern Blot ...

Background: The aim of the study was to determine mechanism Moringa oleifera-induced apoptosis in HeLa cells. cells over-express cyclin E and B1, abrogate G0-G1 G2-M cell cycle arrest, promoting tumorigenesis. Cyclin E, E2F1 telomerase expression, caspase-3 -7 activation were assessed after 24-treatment with M. oleifera leaf fractions. Material methods: Apoptosis through caspase-7 determined qu...

The nature of the colony-forming cell in the bone marrow of patients with monoclonal gammopathy, as defined in the stem cell assay described by Hamburger and Salmon, has been studied. It could be shown that the colony-forming cells produce immunoglobulins of the same idiotype, heavy chain and light chain, as the monoclonal bone marrow cells in the patient. Data regarding the presence or absence...

the purpose of this study is to investigate the effects of quercetin as an inhibitor of heat shock proteins and hyperthermia on the induced dna damages and colony formation ability of du145 tumor spheroid culture. du145 cells were cultured as spheroids. on day11, spheroids with mean diameter 100 μm were treated with different concentration of quercetin for 24 hours and then exposed to hyperther...