A reproducible method for growing normal human bone marrow B-lineage colonies in agar is described. The clonogenic cells require a rich medium, Opti-MEM (GIBCO/BRL, Burlington, Ontario, Canada), and a source of T-cell-derived factors for growth. The conditions described appear to be limiting for the colony progenitor, suggesting assay clonality. Three novel methods that permit routine and rapid...

Hematopoietin-1 (H-1) was purified from the human cell line 5637 and two amino acid sequences were observed in the preparation. One sequence was identical to that of interleukin 1 alpha (IL 1 alpha) and the other to that of IL 1 beta. The action of recombinant IL 1 alpha and other hematopoietic growth factors was studied using (a) a high proliferative potential colony-forming cell assay that us...

Understanding tumor heterogeneity is an urgent and unmet need in cancer research. In this study, we used a morphology-based optical cell separation process to classify a heterogeneous cancer cell population into characteristic subpopulations. To classify the cell subpopulations, we assessed their morphology in hydrogel, a three-dimensional culture environment that induces morphological changes ...

We describe two assays to detect the action of colony-inhibiting cells. In the first assay, we used a simple density separation technique to remove dense neutrophils (PMN) from suspensions of blood and of bone marrow cells prior to culture in semisolid agar. Conditions were arranged to ensure that control suspensions of unseparated cells and test suspensions of buoyant mononuclear cells differe...

Abstract Radiotherapy resistance is a challenge for colorectal cancer (CRC) treatment. Circular RNAs (circRNAs) play vital roles in the occurrence and development of CRC. This study aimed to investigate role circ_0005615 regulating radiosensitivity The levels circ_0005615, microRNA-665 (miR-665), notch receptor 1 (NOTCH1) were detected by quantitative real-time PCR or western blot. CRC cells wa...

Cancer is a major health problem and it has been causing great damage in recent years worldwide. Serious side effects of chemotherapeutics used cancer treatment make natural products valuable. Combined use chemotherapy drugs herbal an important approach reducing revealing more effective treatment. In this study, the methanolic extract from Portulaca oleracea (POM) alone combination with Irinote...

To investigate the effect and molecular mechanism of vitexicarpin on biological behavior colorectal cancer cells is objective study. The human epithelial cell line Caco-2 were divided into control group, low, middle high dose groups vitexicarpin, plasmid cloning DNA-circ_0000419 DNA anti-microRNA-224 anti-microRNA-224-negative vitexicarpin+small interfering-circ_0000419 group vitexicarpin+micro...

Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less w...

This study aimed to test the antimicrobial activity of endophytic bacteria and fungi isolates soursop (Annona muricata L.) in inhibiting growth E.coli, S. epidermidis, C. albicans. The stages research were isolation bacteria, assay, characterization potential fungi. Endophytic microbial was isolated from leaves, bark, root taken Praya, Central Lombok. agar diffusion method used assay. Character...

objective(s) methicillin-resistant staphylococcus aureus (mrsa) is an important cause of nosocomial and community infections. biofilm formation, mediated by a polysaccharide intercellular adhesin (pia) and encoded by the ica operon, is considered to be an important virulence factor in both s. epidermidis and s. aureus. however, the clinical impact of the ica locus and pia production is less wel...