We examined changes in the ultrastructure and localization of major extracellular matrix components, including 5 types of collagen (type I, III, IV, VI, and VIII), laminin, fibronectin, and heparan sulfate proteoglycan in Descemet's membrane of the cornea of diabetic GK rats. In the cornea of diabetic GK rats, more long-spacing collagen fibrils were observed in Descemet's membrane than in the m...

AIMS/BACKGROUND The morphological changes in Bruch's membrane and its constituent collagen seen during aging have been studied extensively but the chemical nature of the collagen and any aging changes have not previously been evaluated. METHODS A method for preparing purified Bruch's membrane from human cadaver eyes by dissection preceded by trypsin digestion was developed. Following pepsin d...

PURPOSE Collagen gels may prove to be potential carriers for transplantation of cultured corneal epithelial cells. The purpose of this study was to evaluate the suitability of collagen gels in comparison with corneal stromal blocks as the substrate to support the growth of human corneal epithelial cells in culture and the synthesis and deposition of the basement membrane components by these cel...

The demonstration that epithelial cells of mouse and rat synthesize basement membrane antigens in tissue culture has established the concept that epithelia in general synthesize basement membrane (Pierce et al., 1964; Pierce and Nakane, 1967). Epithelial basement membranes contain hydroxyproline (Mukerjee et al., 1965). In addition, these membranes contain antigens shared by collagen and other ...

background: guided tissue regeneration (gtr) allows mesenchymal cells to repopulate the defects. however, there is limited information regarding the effi cacy of different membranes. the present study was designed to histologically and histomorphometrically compare three collagen membranes in regenerative treatment of dehiscence defects in dogs. materials and methods: this 8 weeks experimental ...

The efficacy of photodynamic therapy (PDT) depends upon the delivery of both photosensitizing drug and oxygen. In this study, we hypothesized that local vascular microenvironment is a determinant of tumor response to PDT. Tumor vascularization and its basement membrane (collagen) were studied as a function of supplementation with basement membrane matrix (Matrigel) at the time of tumor cell ino...

Objectives To evaluate new lateral bone formation and volume augmentation by guided regeneration (GBR) in chronic non-contained defects with the use of a non-resorbable TiO2-block. Materials methods Three buccal were created each hemimandible eight beagle dogs allowed to heal for 8 weeks before treatment GBR. Each was randomly allocated 4- or 12-week healing time after GBR, three intervention g...

A technique, the "Loma Linda pouch," is introduced for repairing the perforated maxillary sinus membrane during sinus grafting procedures. A collagen membrane is placed against the perforated site and subsequently covers the internal surface of the maxillary sinus. The collagen membrane is then folded along the lateral access window to form a pouch that surrounds and isolates the graft material.

Collagen XVII, a hemidesmosomal component, mediates the adhesion of epidermal keratinocytes to the underlying basement membrane. It exists as a full-length transmembrane protein and a soluble ectodomain that is proteolytically released from the cell surface by sheddases of a disintegrin and metalloproteinase (ADAM) family; TACE, the tumor necrosis factor-alpha-converting enzyme, is the major ph...