An investigative study was carried out to determine the bacteriological and physicochemical qualities of borehole and well water samples in Ijebu-Ode, Southwestern Nigeria. Ten water samples each of borehole and well water sources were collected within the geographical location. Physicochemical parameters were determined using standard methods. The total bacterial count was determined by pour p...

UNLABELLED The distribution of resistance to ampicillin, chloramphenicol, sulfonamides, tetracycline, and streptomycin among coliform in the Gomti river water samples was investigated. The coliform populations were isolated on Mac Conky and eosin methylene blue (EMB) agar plates supplemented with antibiotics. The incidence of resistance among the coliform population varied considerably in diffe...

The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic r...

In the Sierra Nevada Mountain wilderness backcountry, debate has occurred regarding the impact of cattle, pack animals, and humans on microbial contamination of the watershed. Using coliform bacteria as a marker, we hypothesized that water from high alpine watersheds with more frequent human or animal use patterns would have increased risks for presence of potentially harmful microbes. We analy...

Verification of membrane filter total coliform colonies was compared in lauryl tryptose broth, and m-LAC broth primary media and brilliant green-lactose-bile broth and EC broth secondary media. Verification in m-LAC broth yielded the greatest number of aerogenic isolates for both untreated surface water and drinking water samples. Verification in brilliant green-lactose-bile broth increased the...

waterborne outbreaks of pathogenic bacteria from contaminated water are serious threats forpublic health. coliform bacteria have been regarded as one of the most important indicators for monitoringpathogenic bacteria. to address potential pathogenic bacterial outbreaks, the distribution and diversity ofcoliform bacteria in jiahe river, which flows through densely-populated urban area in china, ...

Lauryl sulphate broth, introduced by Cowles (1938) and modified by Mallman and Darby (1941), has been widely used in recent years for the presumptive test for coliform bacteria in water. Lauryl sulphate is, to a certain extent, an inhibitory agent and tends to suppress anaerobic lactose fermenting bacteria. Inhibitory agents in culture media are always subject to suspicion. A substance which ca...

A total of 720 whole, romaine lettuce heads were purchased from retail locations in the Upper Midwest and assessed for coliform and Escherichia coli contamination and for the presence of E. coli O157:H7. During a 16-month period (August 2010 through December 2011), coliform and E. coli counts were enumerated on Petrifilm, and the presence of E. coli O157:H7 and the virulence gene eae was evalua...

AIMS To determine whether antibody to lipopolysaccharide-core (LPS-core) antigen is an important component of the antibody, detected by mixed heat-killed coliform antigen, in urine from patients with suspected urinary tract infection. METHODS LPS-core antigen and mixed heat-killed coliform antigen were used in an enzyme linked immunosorbent assay (ELISA) to measure IgG antibody in midstream u...

AIMS Investigations were carried out to observe the influence of winter/spring and summer periods on the survival of Salmonella typhimurium and indicator bacteria (psychrophilic, mesophilic, coliform and faecal coliform bacteria and faecal streptococci) in the solid fraction of pig slurry from agricultural wastewater treatment plant. METHODS AND RESULTS Leather squares and PVC bottles with op...