By application of modern recombinant DNA technology, especially the polymerase chain reaction (PCR)/dot-blot hybridization techniques, we have investigated the molecular basis of β-thalassemia from four different regions of Iran: central, south-east, south and north. In this study, the DNA samples were isolated from patients and for the identification of the mutations, the 6 oligonucleotide...

In order to study and compare the phosphate transporter gene codon usage and it's respond to the traits like salt tolerance, day length, Pollination and temperature in different plants, 100 isoform from 10 plants are extracted from NCBI website and then analyzed with Gene Infinity and Minitab 16 software. The result shows that the highest codon usage similarity (81.95%) was for wheat a...

Background Human coagulation factor IX is a 57kDa plasma serine protease made in Liver which plays a vital role in the blood coagulation cascade. FIX deficiency causes severe disorder Hemophilia B or Christmas disease. Nowadays, recombinant proteins have important roles in treatment of diseases. Although, cultivated mammalian cells because of their ability for producing properly folded protein ...

conclusions a fusion tag from the pet32 expression plasmid permits the recovery of the recombinant fusion fgf-2 from e. coli, without affecting its biological activity. objectives the objective of this study was to overexpress and purify the high-level active human bfgf in escherichia coli (e. coli). materials and methods this experimental study was conducted in the islamic republic of iran. af...

background: glaucomatous neuropathy is a type of cell death due to apoptosis. the p53 gene is one of the regulatory genes of apoptosis. recently, the association between the p53 gene encoding for proline at codon 72 and primary open-angle glaucoma (poag) has been studied in some ethnic groups. this study is the first association analysis of poag and p53 codon 72 polymorphism in iranian patients...

As one of a dozen monotypic genera in the family Lauraceae, systematic position Dodecadenia Nees remains controversial. Here, two complete plastomes grandiflora were sequenced. The plastid genomes, with length 152,659 bp and 152,773 bp, had similar quadripartite structure. Both consisted large single-copy (LSC) region 93,740 93,791 small (SSC) 18,805 18,846 pair inverted repeats (IR) regions 20...

In order to study and compare the phosphate transporter gene codon usage and it's respond to the traits like salt tolerance, day length, Pollination and temperature in different plants, 100 isoform from 10 plants are extracted from NCBI website and then analyzed with Gene Infinity and Minitab 16 software. The result shows that the highest codon usage similarity (81.95%) was for wheat a...