background: healthcare-associated infections such as nosocomial infections (ni) are important causes of mortality worldwide. objectives in this study we evaluated the nosocomial infections terms of microbiology (resistance, culturing, etc.) in a referral pediatric hospital based on national nosocomial infection surveillance system. patients and methods in an epidemiological surveillance study d...

Absorbed antisera prepared against heat-killed suspensions of a coagulase-negative staphylococcus and of a micrococcus were used in a slide-agglutination test. A collection of 176 coagulase-negative aerobic cocci, from which Staphylococcus aureus had been excluded, was classified into staphylococci and micrococci by means of the test for anaerobic utilization of glucose. Of 113 staphylococci, 9...

The analysis of gel banding patterns of penicillin-binding proteins was used to identify two clinical isolates of a coagulase-negative Staphylococcus species as Staphylococcus caprae, a species originally isolated from goat's milk. One of the isolates was further shown to carry mecA, the structural gene for methicillin resistance.

During 1984, 35 patients undergoing continuous peritoneal dialysis experienced 77 cases of peritonitis with 55 cases (71.4%) related to staphylococci. Coagulase-negative staphylococci were isolated in 41 cases, while Staphylococcus aureus was found in 14. A coexisting tunnel infection was more often associated with S. aureus (7/14) than with coagulase-negative staphylococci (2/41) (P less than ...

A total of 398 consecutive clinical staphylococcus isolates, of which 205 were coagulase negative and 193 were coagulase positive, were tested in parallel by using AutoMicrobic system Gram-Positive Susceptibility-MIC cards and modified Mueller-Hinton agar containing 4% NaCl and oxacillin (6 micrograms/ml). The AutoMicrobic system cards correctly detected 103 of 104 (99%) oxacillin-resistant coa...

Two shuttle vectors containing the gene for protein A (spa) from Staphylococcus aureus have been constructed to study expression of the gene in various strains of S. aureus and in the coagulase-negative species Staphylococcus epidermidis, Staphylococcus capitis, and Staphylococcus xylosus. One plasmid, pSPA15, contains the complete structural gene for protein A, which binds to the cell wall in ...