نتایج جستجو برای: co culturedifferentiationembryonic stem cellin vitro derived germ cellssertoli cell

تعداد نتایج: 2661457  

Journal: :world journal of plastic surgery 0
mohsen hosseinkhani department of anatomical sciences, school of medicine, qazvin university of medical sciences, qazvin, iran davood mehrabani stem cell and transgenic technology research center, department of pathology, shiraz university of medical sciences, shiraz, iran mohammad hassan karimfar department of anatomical sciences, school of medicine, zahedan university of medical sciences, zahedan, iran salar bakhtiyari department of clinical biochemistry, school of medicine, ilam university of medical sciences, ilam, iran amir manafi student research committee, shahid beheshti university of medical sciences, tehran, iran reza shirazi nanobiotechnology research center, avicenna research institute, acecr, tehran, iran

stem cells are self-renewing cells that can be differentiated into other cell types. conventional in vitro models for studying stem cells differentiation are usually preformed in two-dimensional (2d) cultures. the design of three-dimensional (3d) in vitro models which ideally are supposed to mimic the in vivo stem cells microenvironment is potentially useful for inducing stem cell derived tissu...

2018
Fang Fang Zili Li Qian Zhao Honggang Li Chengliang Xiong

Recently, significant progress has been made in ART for the treatment of male infertility. However, current ART has failed to help infertile patients with non-obstructive azoospermia, unless donor sperm is used. In fact, most couples wish to have their own genetically related child. Human induced pluripotent stem cells (hiPSCs) can be generated from patients' somatic cells and in vitro derivati...

Journal: :Journal of cell science 2011
Siim Pauklin Roger A Pedersen Ludovic Vallier

Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of an embryo at the blastocyst stage. They are characterised by the ability to proliferate indefinitely (or selfrenew) in vitro while maintaining pluripotency, i. e. the capacity to differentiate into germ cells and a broad number of cell types that originate from the three primary germ layers endoderm, mesoderm and ectoderm...

Recently new reports have proved the pluripotency of spermatogonial stem cells (SSCs) derived from male gonad. This pluripotent stem cells resembled Embryonic stem cells recognized as Embryonic Stem like cells (ES like cells). ES like cells forms sharp edge colonies that are immunopositive to pluripotency markers and have differentiation capacity to Ectodermal, Mesodermal and Endodermal layers....

Journal: :Stem cells and development 2011
Olympia E Psathaki Karin Hübner Davood Sabour Vittorio Sebastiano Guangming Wu Fumihiro Sugawa Peter Wieacker Petra Pennekamp Hans R Schöler

Germ cells are a unique population of cells responsible for transmitting genetic information from one generation to the next. Our understanding of the key mechanisms underlying germ cell development in vivo remains scarce because of insufficient amounts of cell materials available for conducting biological studies. The establishment of in vitro differentiation models that support the generation...

A Mohammad Eini M Ahmadifar, R Fathi, Y Sharifi

Background Mesenchyme stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and c...

Journal: :avicenna journal of medical biotechnology 0

background: mesenchymal stem cells (mscs) are multipotent cells that can be collected from different sources. under specific conditions, mscs can be differentiated to tissue specific cells in vitro. human umbilical cord mesenchymal stem cells (hucmscs) can easily be harvested and cultured in in vitro conditions. production of germ cells from mesenchymal stem cells is a very interesting and prom...

2016
Wei-Fang Chang Yuh-Ming Hwu Jie Xu Chen-Ju Lin Sheng-Wen Wang An-Sheng Cheng Jean Lu Chung-Hao Lu Li-Ying Sung

Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medicine. In this study, we derived patient-specific iPSCs using CCs from human infertility clinics fo...

2014
Jens Durruthy Durruthy Cyril Ramathal Meena Sukhwani Fang Fang Jun Cui Kyle E. Orwig Renee A. Reijo Pera

Studies of human germ cell development are limited in large part by inaccessibility of germ cells during development. Moreover, although several studies have reported differentiation of mouse and human germ cells from pluripotent stem cells (PSCs) in vitro, differentiation of human germ cells from PSCs in vivo has not been reported. Here, we tested whether mRNA reprogramming in combination with...

Journal: :iranian red crescent medical journal 0
maryam nazm bojnordi department of anatomical sciences, medical sciences faculty, tarbiat modares university, jalale-ale-ahmad highway, p.o. box 14115-175, ir iran +98-2182884503, [email protected] mansoureh movahedin department of anatomical sciences, medical sciences faculty, tarbiat modares university, jalale-ale-ahmad highway, p.o. box 14115-175, ir iran +98-2182884503, [email protected]; department of anatomical sciences, medical sciences faculty, tarbiat modares university, jalale-ale-ahmad highway, p.o. box 14115-175, ir iran +98-2182884503, [email protected] taki tiraihi department of anatomical sciences, medical sciences faculty, tarbiat modares university, jalale-ale-ahmad highway, p.o. box 14115-175, ir iran +98-2182884503, [email protected] mohamad javan department of physiology, medical sciences faculty, tarbiat modares university, ir iran

background new research proposes the pluripotency of spermatogonial cells obtained from testis. these spermatogonia-derived stem cells are called embryonic stem-like cells that express embryonic stem cell markers and differentiate to the three germ layers. objectives the aim of the present study was to generate embryonic stem-like cells from neonatal mouse testis. materials and methods the test...

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