Vitamins k is an important fat-soluble vitamin that can be obtained from plants, bacteria and animals necessary for the blood clotting. It plays a key function as cofactor in synthesizing of clotting proteins liver; recently, interest its functions extra-hepatic tissue has increased. Vitamin deficiency usually caused by abnormal absorption rather than lack food. Apart impact on clotting, chroni...

Blood coagulation reactions are strongly influenced by phospholipids, but little is known about the influence of sphingolipids on coagulation mechanisms. Lysosulfatide (lyso-SF) (sulfogalactosyl sphingosine) prolonged factor Xa (fXa) 1-stage plasma clotting assays, showing it had robust anticoagulant activity. In studies using purified clotting factors, lyso-SF inhibited >90% of prothrombin (II...

COAGULATION DISORDERS have represented useful clotting models. During the past decade new problems have appeared. Several conditions due to structural abnormalities of clotting factors have been described. Several dysfibrinogenemias, hemophilia B and BM variants, and factor X Friuli and other factor X variants are now well-defined clotting disorders. 1-8 Recently, two abnormal factor VII defect...

A wide range of factor VIII and IX levels is observed in heterozygous carriers of hemophilia as well as in noncarriers. In female carriers, extreme lyonization may lead to low clotting factor levels. We studied the effect of heterozygous hemophilia carriership on the occurrence of bleeding symptoms. A postal survey was performed among most of the women who were tested for carriership of hemophi...

ASSAY PRINCIPLE: The method is a clotting assay where all the clotting factors are present (constant and in excess, brought by the deficient plasma), excepted for FII, which is brought by the diluted tested plasma, and clotting is triggered with calcium thromboplastin. FII is the limiting factor and clotting time is inversely proportional to the concentration of FII. There is an inverse linear ...

Inherited diseases might be treated by introducing normal genes into a patient’s somatic tissues to correct the genetic defects. In the case of hemophilia resulting from a missing clotting factor. the required gene could be introduced into any cell as long as active factor reached the circulation. We previously showed that retroviral vectors can efficiently transfer genes into normal skin fibro...

Drummond, Margaret C. (Emory University, Atlanta, Ga.) and Morris Tager. Fibrinogen clotting and fibrino-peptide formation by staphylocoagulase and the coagulase-reacting factor. J. Bacteriol. 85:628-635. 1963.-The mechanism of fibrinogen clotting by staphylocoagulase and the coagulase-reacting factor (CRF) was studied from the standpoint of the products of the reaction, and compared with throm...