Clostridial glucosylating cytotoxins inactivate mammalian Rho GTPases by mono-O glucosylation of a conserved threonine residue located in the switch 1 region of the target protein. Here we report that EhRho1, a RhoA-like GTPase from the protozoan parasite Entamoeba histolytica, is glucosylated by clostridial cytotoxins. Recombinant glutathione S-transferase-EhRho1 and EhRho1 from cell lysate of...

BACKGROUND Clostridium difficile is the major cause of nosocomial antibiotic-associated diarrhoea with the potential risk of progressing to severe clinical outcomes including death. It is not unusual for Clostridium difficile infection to progress to complications of toxic megacolon, bowel perforation and even Gram-negative sepsis following pathological changes in the intestinal mucosa. These c...

The Clostridium-like organisms TO-931T and HD-17, isolated from human faeces, have high levels of bile acid 7alpha-dehydroxylating activity. Sequencing of their 16S rDNA demonstrated that they belong to cluster XI of the genus Clostridium and that they represent a new and distinct line of descent. Clostridium bifermentans and Clostridium sordellii in cluster XI also possess bile acid 7alpha-deh...

An obligate anaerobic, mesophilic, motile and endospore-forming bacterium, designated 1D(T), was isolated from a subsurface soil sample. The young culture of strain 1D(T) was Gram-positive and formed oval spores that were central in position. Based on the biochemical, chemotaxonomic and physiological data, strain 1D(T) appears to be a member of the genus Clostridium. Strain 1D(T) was found to b...

INTRODUCTION Clostridium difficile is the most common cause of healthcare-associated infections and can have devastating morbidity and mortality. Traditional treatment algorithms involve intravenous metronidazole and enteric metronidazole or vancomycin. Fidaxomicin (DificidR) targets "switch regions" within RNA polymerases and effectively kills clostridium difficile bacteria and is typically ad...

The reactivity of a commercial latex test with thirty-three species of bacteria was tested. Toxigenic and nontoxigenic strains of Clostridium difficile gave a positive result in the CD D-1 latex test. Cross-reactions were also given by C. putrificum, C. sporogenes and proteolytic C. botulinum.

conclusions immediate diagnosis based on careful history and physical examination are essential for management of botulism. people should be notified about proper food handling and preparation of traditional homemade foods. patients and methods during an outbreak, 10 members of a family referred to the hospital because of food poisoning. all patients had a history of consumption of doogh, a tra...

clostridium diffcile is part of the normal intestinal flora in a small proportion of healthy persons. when exposed to antibiotics, the normal enteric flora is altered, permitting the overgrowth of these relatively resistant organisms. proliferation of the organisms with localized production of their toxins leads to disease (pseudomembran colitis or antibiotic associated diarrhea). in this study...

An amplified fragment length polymorphism (AFLP) method was applied to 129 strains representing 24 different Clostridium species, with special emphasis on pathogenic clostridia of medical or veterinary interest, to assess the potential of AFLP for identification of clostridia. In addition, the ability of the same AFLP protocol to type clostridia at the strain level was assessed by focusing on C...