Peripheral blood stem cells (PBSCs) are being used as an alternative to autologous marrow rescue for hematopoietic reconstitution after high-dose chemotherapy in patients with neuroblastoma and other solid malignancies. Use of PBSCs is preferred by some because of the belief that there is less risk of tumor contamination. Because tumor stem cell contamination is thought to be one contributing c...

PURPOSE To determine the chronic low-dose radiation effects caused by α-particle radiation from (226)Ra over multiple cell generations in CHSE/F fish cells and HaCaT human cells. METHODS CHSE/F cells and HaCaT cells were cultured in medium containing (226)Ra to deliver the chronic low-dose α-particle radiation. Clonogenic assay was used to test the clonogenic survival fractions of cells with ...

Cryptocarya pulchrinervia is an Indonesian indigenous plant that grows in Sumatra, Kalimantan and Papua. One of the new compounds extracted from this was cryptobrachytone C, which known to be cytotoxic against cancer cells Murine leukemia P388 with IC50 10.52 ?M. In study, cytotoxicity anticancer properties C on proliferation, apoptosis, migration clone formation MCF-7 T47D breast cell lines we...

The cellular toxicity of three types of carbon nanoparticles, namely HiPco single-walled carbon nanotubes (SWCNT), arc discharge SWCNT and Printex 90 carbon black nanoparticles, was studied on three different cell models including the human alveolar carcinoma epithelial cell line (A549), the normal human bronchial epithelial cell line (BEAS-2B) and the human keratinocyte cell line (HaCaT) using...

BACKGROUND Hyperthermia is used in combination with radiotherapy and/or chemotherapy in the treatment of various types of cancer. Currently, the tumor cell response to hyperthermia is determined largely based on the size reduction of tumor mass, which is insensitive. METHODS We tested the feasibility of bioluminescent imaging (BLI) in evaluation of the tumor cell response to hyperthermia by e...

Background/Aim: This study was designed to investigate the effect of IL-39 on T24 bladder cancer (BC) cell line survival and growth. Materials Methods: In order assess direct survival, proliferation, apoptosis BC cells, we utilized a clonogenic assay, proliferation caspase-3 activity kits. Potential proliferative apoptotic molecular mechanisms were evaluated by RT-PCR. Results: Treatment cells ...

Tumor contamination of hematopoietic stem cell grafts may influence the outcome of breast cancer patients treated with high-dose chemotherapy. The goals of this study were: (a) to evaluate the prevalence of tumor contamination of bone marrow (BM) harvests in patients responding to systemic chemotherapy; (b) to evaluate reduction of BM tumor contamination by ex vivo purging with 4-hydroperoxycyc...

In a murine squamous cell carcinoma (SCC) model, we have demonstrated that both 1,25-dihydroxycholecalciferol (1,25-D3) and the analogue 1,25-dihydroxy-16-ene-23-yne-cholecalciferol (Ro23-7553) have significant in vitro and in vivo antitumor activity. We have examined here the cell cycle effect of 1,25-D3 and Ro23-7553 on SCCVII/SF tumor cells by quantitating nuclear DNA using a detergent-tryps...