نتایج جستجو برای: cleavage sites

تعداد نتایج: 320451  

Journal: :Biochemistry 2001
B D Lovett D Strumberg I A Blair S Pang D A Burden M D Megonigal E F Rappaport T R Rebbeck N Osheroff Y G Pommier C A Felix

Chromosomal breakage resulting from stabilization of DNA topoisomerase II covalent complexes by epipodophyllotoxins may play a role in the genesis of leukemia-associated MLL gene translocations. We investigated whether etoposide catechol and quinone metabolites can damage the MLL breakpoint cluster region in a DNA topoisomerase II-dependent manner like the parent drug and the nature of the dama...

Journal: :Nucleic acids research 1990
G Capranico K W Kohn Y Pommier

Doxorubicin, a DNA-intercalator, is one of several anti-cancer drugs that have been found to stabilizes topoisomerase II cleavage complexes at drug-specific DNA sites. The distribution and DNA sequence environments of doxorubicin-stabilized sites were determined in the SV40 genome. The sites were found to be most concentrated in the major nuclear matrix-associated region and nearly absent in th...

Journal: :Cell 2008
Sami Mahrus Jonathan C. Trinidad David T. Barkan Andrej Sali Alma L. Burlingame James A. Wells

The nearly 600 proteases in the human genome regulate a diversity of biological processes, including programmed cell death. Comprehensive characterization of protease signaling in complex biological samples is limited by available proteomic methods. We have developed a general approach for global identification of proteolytic cleavage sites using an engineered enzyme to selectively biotinylate ...

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